Nalm-6 Cells
Product number:
300297
Insights on the Nalm-6 cell line
Description | The Nalm-6 cell line, derived from the peripheral blood of a patient with B-cell precursor acute lymphoblastic leukemia (ALL), has become a critical tool in leukemia research. The human cell line Nalm 6 encapsulates the biological characteristics of B-cell ALL, providing a unique window into the disease's genomic landscape, including genome instability and DNA repair mechanisms. The utility of Nalm-6 cells extends to studying the efficacy of available therapeutic targets and existing resistance mechanisms. The cell line's sensitivity to cytotoxic agents and its role in elucidating the homologous recombination (HDR) repair functions are of particular interest, especially concerning the HDR cells' ability to correct DNA damage. The Nalm6 cell line is a reliable model for studying the complex nature of acute leukemia. It facilitates research into the gene expression profiles involved in glycolysis, lipid and carbohydrates metabolism, and the mTORC1 pathway, highlighting the metabolic reprogramming in leukemia cells. Furthermore, the cell line's application in reverse genetics and whole transcriptome analysis aids in dissecting the intricate molecular networks driving leukemia progression and resistance. Research utilizing the Nalm-6 cell line, including studies on clonal variants like clone G5 and resistant cell lines such as those with a high HPRT mutation frequency or C9 with resistance index, provides insights into leukemia's heterogeneity. The exploration of leukemia dynamics, especially in the context of glucocorticoid resistance and MSH2 expression, underscores the potential for developing more targeted and effective treatments for ALL. In summary, the Nalm-6 cell line is a pivotal resource in leukemia research, offering profound insights into B-cell ALL through its applications in studying genomic instability, DNA repair mechanisms, therapeutic target efficacy, resistance mechanisms, and the underlying molecular pathways influencing leukemia's complex biology and heterogeneity. |
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Organism | Human |
Tissue | Blood |
Disease | Adult B acute lymphoblastic leukemia |
Synonyms | NALM-6, NALM 6, Nalm 6, NALM6, Nalm6, NALM-6-M1 |
Details of the lymphoblastic leukemia cell line Nalm-6
Age | 19 years |
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Gender | Male |
Morphology | Round cells |
Cell type | B cell precursor |
Growth properties | Suspension |
Documentation
Citation | Nalm-6 (Cytion catalog number 300297) |
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Biosafety level | 1 |
Genomics
Reverse transcriptase | Negative |
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Handling Nalm6 cells
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 35 to 40 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | Nalm-6 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 13
D13S317: 9,13
D16S539: 10,11
D5S818: 11,12
D7S820: 8,11
TH01: 8,9
TPOX: 8,9
vWA: 15,16
D3S1358: 16
D21S11: 29
D18S51: 12,15
Penta E: 11
Penta D: 9,14
D8S1179: 12,13
FGA: 22,24
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