DU4475 Cells
General information
Organism | Human |
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Tissue | Breast |
Disease | Breast carcinoma |
Metastatic site | Skin |
Applications | 3D cell culture, Immuno-oncology |
Synonyms | Du4475, DU-4475, Du-4475, DU 4475, Du 4475, Duke University 4475 |
Characteristics
Age | 62 years |
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Gender | Female |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Clusters in Suspension |
Identifiers / Biosafety / Citation
Citation | DU4475 (Cytion catalog number 300371) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | AK-1, 1, ES-D, 1, G6PD, B, GLO-I, 2, Me-2, 2, PGM1, 1-2, PGM3, 1 |
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Tumorigenic | Yes, in nude mice |
Viruses | EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Karyotype | human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93xxxx, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 - sideline with -20, -20, +del(7)(p11) - gain of 1q and loss of 6q typical in breast carcinoma - resembles published karyotype |
Handling
Culture Medium | RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3 (Cytion article number 820702a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 9,12
D13S317: 11,14
D16S539: 11,12
D5S818: 11
D7S820: 9,10
TH01: 6,8
TPOX: 8
vWA: 17
D3S1358: 14,16
D21S11: 29,31.2
D18S51: 14,16
Penta E: 7,13
Penta D: 13,14
D8S1179: 10,13
FGA: 22,25
D6S1043: 11
D2S1338: 20,25
D12S391: 18.3,25
D19S433: 14
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