ES-2 Cells
General information
Description | The tumor was described as a poorly differentiated ovarian clear cell carcinoma.Initially, the cells were grown in soft agar.The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin(MRA-CN).ES-2 cells express low levels of P glycoprotein. |
---|---|
Organism | Human |
Tissue | Ovary |
Disease | Ovarian clear cell adenocarcinoma |
Synonyms | ES2 |
Characteristics
Age | 47 years |
---|---|
Gender | Female |
Ethnicity | European |
Morphology | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | ES-2 (Cytion catalog number 305038) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Protein expression | P Glycoprotein |
---|---|
Tumorigenic | Yes |
Handling
Culture Medium | McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 10,15
D13S317: 11
D16S539: 11,13
D5S818: 11,13
D7S820: 11
TH01: 9.3
TPOX: 8,12
vWA: 16,17
D3S1358: 15,18
D21S11: 32.2,33.2
D18S51: 13,15
Penta E: 13,16
Penta D: 8,13
D8S1179: 14
FGA: 21
D6S1043: 11,12
D2S1338: 17,23
D12S391: 20,21
D19S433: 15,15.2
|