Daoy Cells
General information
Description | The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy. |
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Organism | Human |
Tissue | Brain, cerebellum |
Disease | Medulloblastoma |
Synonyms | DAOY, D324 Med, D-324 Med, D324 MED, D-324MED, D324 |
Characteristics
Age | 4 years |
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Gender | Male |
Ethnicity | European |
Morphology | Polygonal |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | Daoy (Cytion catalog number 305053) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 34 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 13,14
D16S539: 10
D5S818: 11,13
D7S820: 8,10
TH01: 9
TPOX: 8,10
vWA: 14,20
D3S1358: 15
D21S11: 29,31.2
D18S51: 12
Penta E: 7,11
Penta D: 10,13
D8S1179: 13,15
FGA: 23
D1S1656: 17.3
D6S1043: 12
D2S1338: 29,31.2
D12S391: 20
D19S433: 14.2
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