B-CPAP Cells
General information
Description | B-CPAP is a human papillary thyroid carcinoma cell line that was established from the primary tumor of a 74-year-old woman. The cell line exhibits epithelial-like morphology and is commonly used in research to study thyroid cancer biology, including mechanisms of tumorigenesis and metastasis. B-CPAP cells are notable for harboring a BRAF V600E mutation, which is a common genetic alteration associated with aggressive thyroid cancers and serves as a critical model for evaluating BRAF inhibitors as therapeutic agents. In addition to the BRAF mutation, B-CPAP cells express thyroid-specific markers such as thyroglobulin and thyroid-stimulating hormone receptor, making them a valuable model for studying thyroid gland function and pathology. They have been extensively used in studies investigating the signaling pathways involved in thyroid cancer progression, including MAPK/ERK pathway activation. These cells are also employed in drug resistance and apoptosis studies, providing insights into the mechanisms that might underpin therapeutic failures in thyroid cancer treatments. |
---|---|
Organism | Human |
Tissue | Thyroid |
Disease | Thyroid carcinoma |
Synonyms | BC-PAP, BCPAP |
Characteristics
Age | 76 years |
---|---|
Gender | Female |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | B-CPAP (Cytion catalog number 305081) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 13
D13S317: 12
D16S539: 11,12
D5S818: 10,11
D7S820: 10
TH01: 6,9.3
TPOX: 8,11
vWA: 14,17
D3S1358: 16,17
D21S11: 30,31.2
D18S51: 13,17
Penta E: 5,12
Penta D: 10,11
D8S1179: 12,13
FGA: 20,23
D6S1043: 12,19
D2S1338: 18
D12S391: 18,23
D19S433: 13.2,15
|