NCI-H524 Cells
Product number:
305120
General information
Description | The line was established in 1982 from metastatic lymph nodes of a 63-year-old male Caucasian smoker with non-small cell lung carcinoma. The patient received prior chemotherapy and radiation therapy. |
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Organism | Human |
Tissue | Lung |
Disease | Lung small cell carcinoma |
Metastatic site | Lymph Nodes |
Synonyms | NCI-H524 , H-524, NCIH524 |
Characteristics
Age | 63 years |
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Gender | Male |
Ethnicity | European |
Morphology | Rounded |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | NCI-H524 (Cytion catalog number 305120) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 100 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | 1?10^5 to 1?10^6 cells/mL |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | NCI-H524 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 11,12
TH01: 8,9.3
TPOX: 8,10
vWA: 14,17
D3S1358: 15
D21S11: 29,30
D18S51: 12,13
Penta E: 5,15
Penta D: 12,13
D8S1179: 13,15
FGA: 21,25
D6S1043: 11,13
D2S1338: 16,17
D12S391: 16,21
D19S433: 16
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