D341Med Cells
General information
Description | The D341 Med cell line was established in 1988 by Friedman et al. from tumor tissue taken from a boy with medulloblastoma. |
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Organism | Human |
Tissue | Brain, cerebellum |
Disease | Medulloblastoma |
Synonyms | D-341 Med, D-341 MED, D-341MED, D341_Med, D341Med, D341MED, D341MD, D-341, D341, Med 341, H341 |
Characteristics
Age | 3,5 years |
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Gender | Male |
Ethnicity | European |
Morphology | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | D341Med (Cytion catalog number 305136) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | glutamine synthetase positive, neuron specific enolase positive, glial fibrillary acidic proteins negative, S100 (S-100) protein negative, neuroectodermal antigen positive, recognized by the UJ13A monoclonal antibody |
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Tumorigenic | Yes |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 37 hours |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | 1:3 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 9,10,11
D13S317: 11,13
D16S539: 12,14
D5S818: 11,12
D7S820: 9,13
TH01: 6,9.3
TPOX: 8,11
vWA: 17,18
D3S1358: 16,18
D21S11: 30,31
D18S51: 12,17
Penta E: 8,15
Penta D: 9,13
D8S1179: 14
FGA: 19,23
D6S1043: 12,19
D2S1338: 17
D12S391: 17,18,24
D19S433: 13
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