NRK Cells
General information
Description | The NRK cell line, derived from a Rattus norvegicus (rat) kidney, is an invaluable tool in biological research. These cells possess an epithelial morphology, meaning they form sheets covering the organs' surfaces and protecting against foreign substances. In the case of the kidneys, the epithelial cells play a crucial role in the storage and subsequent secretion of excretory materials. This makes the NRK cell line particularly suitable for studying renal physiology. By utilizing these cells, researchers can investigate the intricate processes involved in kidney function and gain insights into various aspects of renal physiology. Moreover, the NRK cell line is not limited to studying renal physiology alone. These versatile cells can also be employed in cancer research. Their epithelial morphology and origin from a normal rat kidney make them an excellent model for investigating the behaviour and characteristics of cancer cells in a controlled environment. One application that leverages the unique properties of NRK cells is 3D cell culture. This technique involves growing cells in a three-dimensional matrix miming the natural cellular environment more closely than traditional two-dimensional culture. NRK cells can be cultured in this manner, allowing researchers to create complex tissue models that closely resemble the native structure of the kidney. This facilitates the study of cellular behaviour, interactions, and responses in a more physiologically relevant context. |
---|---|
Organism | Rat |
Tissue | Kidney |
Synonyms | Normal Rat Kidney |
Characteristics
Age | Adult |
---|---|
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NRK (Cytion catalog number 305195) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|