4T1

€340.00*

Prices excl. VAT plus shipping costs

Product number: 300300

Description	The 4T1 cell line is a widely used model in cancer research due to its high similarity to human breast cancer. Derived from a BALB/c mouse, the tumor growth and metastatic spread of 4T1 cells closely mimic the behavior of human breast cancer. The 4T1 cell line is considered an animal model for stage IV human breast cancer, as it is able to spontaneously metastasize in both post-operative and non-surgical models. This makes the 4T1-induced tumors a valuable tool for studying the progression and metastasis of breast cancer, as well as the efficacy of various treatments. When injected into BALB/c mice, 4T1 cells spontaneously produce highly metastatic tumors that can spread to various organs such as the lung, liver, lymph nodes, and brain, while the primary tumor continues to grow in situ. Furthermore, 4T1 cells are resistant to 6-thioguanine, which allows for the detection of micro-metastatic cells (as few as 1) with greater accuracy than most other tumor models. This means that there is no need to count nodules or weigh target organs. In addition, the 4T1 cell line has been used to study the molecular and cellular mechanisms underlying breast cancer progression and metastasis, including the role of signaling pathways, gene expression, and the tumor microenvironment. Overall, the 4T1 cell line is a valuable tool for the study of breast cancer biology and the development of new treatments.
Organism	Mouse
Tissue	Breast, mammary gland
Disease	Malignant neoplasm
Applications	4T1 cells accurately imitate the characteristics of human breast cancer in its most advanced stage - Stage IV.
Synonyms	4T1-A, 4T1.0, 4T1/WT

Gender	Female
Morphology	Epithelial
Growth properties	Adherent

Identifiers / Biosafety / Citation

Citation	4T1 (CLS catalog number 300300)
Biosafety level	1

Expression / Mutation / Susceptibility

Tumorigenic	Yes, in BALB/c mice.

Culture Medium	RPMI 1640
Medium supplements	10% FBS, 2.0 mM L-glutamin, 2.0 g/L L-glucose, 2.0 g/L NaHCO3
Passaging solution	Accutase
Freeze medium	CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Quality control / Genetic profile / HLA

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Bulk order

Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.