6T-CEM

€340.00*

Prices excl. VAT plus shipping costs

Product number: 305132

Description	The 6T-CEM cell line is a thioguanine-resistant variant of the CCRF-CEM human T-cell leukemia cell line. These cells are deficient in the enzyme hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) which confers resistance to thioguanine. These cells are also known for secreting high levels of an immunosuppressive factor. The 6T-CEM cells have been widely used as a fusion partner for creating human T-cell hybridomas. Additionally, 6T-CEM cells are a suspension culture, which means they grow in a liquid medium and can be maintained by the addition or replacement of fresh medium. Overall, 6T-CEM cells are a useful tool for researchers studying T-cell acute lymphoblastic leukemia, drug resistance, and the development of new treatment strategies and the molecular pathways involved in leukemogenesis, as well as for the generation of human T-cell hybridomas for immunological research.
Organism	Human
Tissue	Peripheral blood
Disease	T-cell acute lymphoblastic leukemia
Synonyms	6-T CEM

Age	4 years
Gender	Female
Ethnicity	Asian
Morphology	Lymphoblast
Growth properties	Suspension

Identifiers / Biosafety / Citation

Citation	6T-CEM (CLS catalog number 305132)
Biosafety level	2

Expression / Mutation / Susceptibility

Culture Medium	Alpha MEM
Medium supplements	10% FBS, 2.7 mM L-glutamine, 1.0 g/L glucose, 2.2 g/L NaHCO3
Passaging solution	The utilization of a passaging solution is not necessary when passaging cells that are cultured in suspension. The appropriate procedure is to dilute the cells in accordance with the indicated guidelines.
Subculturing	Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Quality control / Genetic profile / HLA

Sterility	Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.
STR profile	Amelogenin: X,X, CSF1PO: 10,11 D13S317: 11,12 D16S539: 10,13 D5S818: 11,13 D7S820: 9,14 TH01: 6,7 TPOX: 8 vWA: 17,19 D3S1358: 15 D21S11: 31,33.2 D18S51: 13,18 Penta E: 5,14 Penta D: 11 D8S1179: 13 FGA: 23,24 D6S1043: 11,14 D2S1338: 24 D12S391: 17,18,20,21 D19S433: 14,15

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Bulk order

Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.