AE-1 Cells
Product number:
300635
General information
Description | Derived from a fusion of Mus musculus B lymphocytes and myeloma cells, AE-1 hybridoma cells possess lymphoblast morphology and grow in suspension culture. The derivation of AE-1 cells involves immunizing animals with purified human erythrocyte acetylcholinesterase, followed by fusion with Sp2/0-Ag14 myeloma cells. AE-1 cells are specifically designed to target the antigenic determinant of human acetylcholinesterase. They express immunoglobulin and a monoclonal antibody against this enzyme, providing researchers with a powerful tool to investigate the immunological aspects related to acetylcholinesterase in humans. The isotype of AE-1 cells is IgG1, enhancing their binding affinity and functionality. These cells offer remarkable antibody specificity, reacting with human and monkey acetylcholinesterase but binding to a different epitope compared to AE-2 cells. This specificity enables precise targeting and evaluation of acetylcholinesterase-related processes in different species. In the field of immunology, AE-1 cells find various applications. Researchers can utilize them to study immune responses, antibody production, and antigen-antibody interactions. These cells play a crucial role in advancing areas such as vaccine development, autoimmune disease research, and immunotherapy. |
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Organism | Mouse |
Tissue | Hybridoma |
Applications | Immunology, production of therapeutic antibodies |
Synonyms | AE1 |
Characteristics
Morphology | Lymphoblast |
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Cell type | Hybridoma (Spleen, B cell) |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | AE-1 (Cytion catalog number 300635) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Monoclonal antibody isotype: IgG1 against human ACHE (UniProtKB P22303) |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | AE-1 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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