|Description||AE-2 cells are a specialized type of hybridoma animal cells derived from Mus musculus, specifically from B lymphocytes of mice. These lymphoblast cells are essential for various immunology applications and offer exceptional growth properties in suspension culture. AE-2 cells result from the fusion between spleen cells from mice immunized with purified human erythrocyte acetylcholinesterase and Sp2/0-Ag14 myeloma cells. The critical feature of AE-2 cells lies in their ability to express genes that encode immunoglobulin, monoclonal antibodies specifically targeting human acetylcholinesterase. These antibodies belong to the IgG1 isotype, which is highly valuable for immunological studies. AE-2 cells exhibit a lymphoblast morphology characterized by their lymphocyte-like appearance with enhanced growth potential. Their suspension growth properties make them well-suited for large-scale cultures and downstream applications. Immunology researchers will find AE-2 cells particularly useful in various applications. These include antibody production, antigen detection, protein analysis, and other immunological assays. The monoclonal antibodies expressed by AE-2 cells specifically target acetylcholinesterase in human samples, enabling researchers to study its interactions, functions, and potential therapeutic implications.|
|Applications||Immunology, production of therapeutic antibodies|
|Cell type||Hybridoma (Spleen, B cell)|
Identifiers / Biosafety / Citation
|Citation||AE-2 (CLS catalog number 300638)|
Expression / Mutation
|Protein expression||Monoclonal antibody isotype: IgG1 against human ACHE (UniProtKB P22303)|
|Viruses||Negative for mousepox|
|Medium supplements||10% FBS, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate|
|Passaging solution||The utilization of a passaging solution is not necessary when passaging cells that are cultured in suspension. The appropriate procedure is to dilute the cells in accordance with the indicated guidelines.|
|Subculturing||Resuspend cell suspension in the flask and take representative aliquote to count the cell number per ml. Dilute cell suspension to 1 x 10^5 cells/ml with fresh medium and transfer into new flasks.|
|Freeze medium||CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)|
|Handling of cryopreserved cultures||The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.|
|Handling of proliferating cultures||One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.|
Quality control / Genetic profile / HLA
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.