B-LCL-HROC112 genomic DNA – 5 microgram

€300.00*

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Product number: 302023GD5
Product information "B-LCL-HROC112 genomic DNA – 5 microgram"

Manufacturing method

The genomic DNA (gDNA) is isolated by cell lysis followed by the addition of Proteinase K and RNAse A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process. The fragment length of the purified DNA is up to 50kb.

Applications

  • PCR / RT-PCR / qPCR
  • Next-generation sequencing (NGS)
  • Single nucleotide polymorphism (SNP) analysis
  • Genomic analysis
  • Gene expression studies
  • Southern Blot

Concentration

50-100 ng/µl in TE Buffer (10 mM Tris-CL, 0.5 mM EDTA, pH 8.0). If you require a specific concentration, please contact us.

Quality control

  • The quality and purity of the DNA are tested with a spectrophotometer. Absorption at A260/280 is between 1.8 and 2.0. Contamination with RNA, proteoglycans, and polysaccharides is excluded.
  • We can quantify the double-stranded DNA (dsDNA) content free of charge, please contact us for more information.
  • Please contact us for further information if you require DNA from HLA-typed cell lines.
  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Storage

  • Store at 4°C for frequent use or at -20°C for occasional use.
  • For prolonged storage (> 6 months) we recommend -80°C.
  • Avoid more than three freeze/thaw cycles.

Note

Please centrifuge before opening the vial.

Benötigte Produkte

RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The unique composition of this RPMI formulation comprises 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and phenol red.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3, w/o: Phenol red
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The distinctive formulation of this RPMI includes a concentration of 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and is free from phenol red.
Quality Control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
Freeze Medium CM-1 - 500 ml
Cryopreservation Media Variants: 500 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.

Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

Variants from €59.00*
€450.00*
Freeze Medium CM-1 - 100 ml
Cryopreservation Media Variants: 100 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.

Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

Variants from €59.00*
€99.00*
Freeze Medium CM-1 - 50 ml
Cryopreservation Media Variants: 50 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.

Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

€59.00*
Freeze medium CM-ACF - serum free - 500 ml
Cryopreservation Media Variants: 500 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.

Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

Variants from €59.00*
€450.00*
Freeze medium CM-ACF - serum free - 100 ml
Cryopreservation Media Variants: 100 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.

Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

Variants from €59.00*
€99.00*
Freeze medium CM-ACF - serum free - 50 ml
Cryopreservation Media Variants: 50 ml
Long-term storage
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-ACF enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-ACF, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-ACF is a serum-free, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced, serum-free cell freezing medium CM-ACF is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized serum-free ingredients
CM-ACF does not contain serum products. Serum-containing cryopreservation mediums have the disadvantage of fluctuating recovery rates and unclear composition. Since the composition and concentration of proteins and other biological components vary from batch to batch in serum, the reproducibility of experiments with cells that were frozen in a serum-containing medium may be compromised. As each component of CM-ACF is carefully defined, you can rest assured that cells always recover identically.

Contains DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6

Universal
- even for stem cell preservation
All common cell lines can be frozen and thawed to yield many viable cells. Compared to standard media, the rate of recovery of even the most delicate cells is significantly higher. Using CM-ACF, we store over 1000 different cell lines with outstanding success.
Applications & Validation
The cells preserved in our CM-ACF freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.

€59.00*
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Custom projects
Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.