B16-F0 Cells
General information
Description | The B16-F10 cell line is a well-characterized murine melanoma model derived from the skin tissue of a C57BL/6 mouse. It is a subline generated through the 10th serial passage of the original B16 parent tumor line in C57BL/6 mice. This subclone is noted for its high metastatic potential in vivo and is often used in preclinical studies to evaluate the efficacy of immuno-oncology agents and the development of novel therapeutics. The B16-F10 model is used for investigations into tumor growth kinetics, blood and lymphatic vascularization, tumor and immune cell features, and the spontaneous development of metastases, thereby facilitating the development of more effective treatment strategies for melanoma. The murine melanoma cell line B16-F10 grows adherently and displays an epithelial morphology. When implanted intradermally in C57BL/6 mice, the cells form lung colonies. The B16 tumor line, including B16-F10 cells, has been vital in studying local tumor growth and metastasis. B16F10 cell line's role extends to the analysis of protein expression, notably in the context of VEGF, which shows perivascular predominance upon tumor implantation, fostering new blood vessel growth. In summary, the B16-F10 murine melanoma cell line is recognized for its high metastatic potential and serves as a crucial model in preclinical studies for evaluating immune-oncology therapies and novel treatments. |
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Organism | Mouse |
Tissue | Skin |
Disease | Mouse melanoma |
Synonyms | B16/F0, B16F0 |
Characteristics
Gender | Male |
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Morphology | mixture of spindle-shaped and epithelial-like cells |
Cell type | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | B16-F0 (Cytion catalog number 300308) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in syngeneic mice |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | B16-F0 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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