B16-F10 Cells
Key points about B16-F10 cells
Description | The murine melanoma cell line B16-F10 is a subline of the B16 tumour line that was derived from the skin tissue of a C57BL/6J mouse. These cells exhibit a spindle-shaped and epithelial-like morphology and are often used in skin cancer research. Several sublines, for instance, B16-F1 and B16-F10, were derived from the mother B16 line by selection for their ability to form lung colonies in vivo after intravenous injection and subsequently established in vitro after one (B16-F1) or 10 (B16-F10) cycles of lung colony formation. The cell size at rest is approximately 15.4 ?m with no statistical difference between B16-F1 and B16-F10 cells. The doubling times of B16-F10 cells are approximately 20.1 hours. B16-F10 possesses high lung metastatic ability, whereas B16-F1 is a subline with low metastatic potential. B16-F10 melanoma cells have been widely used as a poorly immunogenic and highly aggressive model for murine tumor immunotherapy studies. Invasive analysis shows that B16-4A5 is the most aggressive melanoma cell line for C57BL/6J's skin, followed by B16-F10 and a diminished aggressive growth pattern by the B16-GMCSF and B16-FLT3 cell lines. Furthermore, melanin is released excessively by the tumor cells, and many metastases in lung and liver parenchyma were found in studies. Regarding protein expression, inoculation of B16-F10 in mouse skin showed a perivascular predominance of VEGF expression on many vessels. VEGF is a signal protein stimulating the growth of new blood vessels. In B16-F10 inoculated test groups, S100, a protein associated with melanomas, was found heterogeneous with moderate to intense positivity. |
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Organism | Mouse |
Tissue | Skin |
Synonyms | B16/F10, B16 F10, B16F10, B16 melanoma F10 |
Features of the B16-F10 cell line
Gender | Male |
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Morphology | Mixture of spindle-shaped and epithelial-like cells |
Growth properties | Adherent |
Specifications
Citation | B16-F10 (Cytion catalog number 305157) |
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Biosafety level | 1 |
Genetic profile of the B16 melanoma model
Culturing methods
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality verification
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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