BALB/3T3 clone A31 Cells
Product number:
305155
General information
Description | S.A. Aaronson and G.T. Todaro created the BALB/3T3 clone A31 cell line in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos. Some evidence suggests that these cells are multipotential mesenchymal cells that can differentiate into different tissues in response to different microenvironmental influences or culture conditions. Mouse embryo cells grown in culture are likely to become permanent cell lines. Repeatedly transferred before confluence to minimize cell-cell contact, the emerging lines are susceptible to contact inhibition of cell division, grow at a high dilution, and exhibit a low saturation density. These cells have a karyotype with a modal number of 78 and a range of 62 to 109. The majority of the cells only had telocentric or acrocentric chromosomes. Some cell lines have been reported to have cytogenetic instability in the literature. Although the BALB/3T3 clone A31 cells are not tumorigenic, they exhibit tumorigenic properties in a semisolid medium. In tissue culture, these cells are highly susceptible to transformation by the oncogenic DNA virus SV40 and the murine sarcoma virus. They were also tested for ectromelia virus and found negative (mousepox). |
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Organism | Mouse |
Tissue | Embryo |
Synonyms | BALB/c 3T3 clone A31, Balb/c3T3, BALB/c 3T3, Balb/c 3T3, BALB/3T3, Balb/3T3-4-Cl31, 3T3 clone A31, BALB/3T3 cl. A31, BALB 3T3 clone A31, BALB/3T3 (clone A31), B/C3T3, 3T3-A31, 3T3(A31), A31, A31N |
Characteristics
Age | Embryo, 14 to 17 days gestation |
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Morphology | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | BALB/3T3 clone A31 (Cytion catalog number 305155) |
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Biosafety level | 2 |
Expression / Mutation
Tumorigenic | No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | BALB/3T3 clone A31 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
M_18-3: 18
M_4-2: 21.3
M_6-7: 12
M_3-2: 14
M_19-2: 14
M_7-1: 25.2
M_1-1: 16
M_Sex: x
M_8-1: 13
M_2-1: 11,16
M_15-3: 22.3
M_6-4: 18
M_11-2: 17
M_1-2: 17
M_17-2: 15,16
M_12-1: 16
M_5-5: 14
M_X-1: 25
M_13-1: 15.2,16.2
Human D4/D8: -
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