|Description||The BEAS-2B cell line derived from bronchial epithelium has been extensively utilized as an in vitro model for respiratory illnesses like lung carcinogenesis. BEAS-2B cells may represent mesenchymal stem cells (MSCs) rather than epithelial cells. Previous research revealed that it maintains similar surface marker expression and osteogenic and adipogenic differentiation capability as an HMSC cell line. BEAS-2B cells have also been used as an in vitro model for epithelial function and pulmonary toxicology, although it was shown that FBS in their growth media modifies their phenotype, reactivity to toxicants, and gene expression in several biological pathways, including carcinogenesis and energy metabolism. The identification of mesenchymal features in BEAS-2B cells suggests that this cell line could serve as a model for hMSC quality control research. Further characterization and validation are required to fully comprehend its mesenchymal nature and its significance for future research. The influence of FBS on the phenotypic and toxicant response of BEAS-2B must be evaluated during study design and interpretation.|
|Synonyms||Beas-2B, BEAS 2B, BEAS2B, Beas2B, Bronchial Epithelium transformed with Ad12-SV40 2B|
Identifiers / Biosafety / Citation
|Citation||BEAS-2B (CLS catalog number 300311)|
Expression / Mutation / Susceptibility
|Viruses||Ad12-SV40 hybrid virus|
|Products||keratins, SV-40 T antigen|
|Freeze medium||CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)|
|Handling of cryopreserved cultures||The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.|
|Handling of proliferating cultures||One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.|
Quality control / Genetic profile / HLA
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.