BV2

€375.00*

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Product number: 305156

Safety data sheet

Product sheet

Description	BV2 cells are a unique type of microglial cells derived from C57/BL6 murine, a widely used laboratory mouse strain. These cells have been immortalized using the v-raf/v-myc carrying J2 retrovirus, resulting in a stable cell line with unique characteristics. BV2 cells express nuclear v-myc and cytoplasmic v-RAF oncogene products and the env gp70 antigen on their surface. One of the critical advantages of BV2 cells is their ability to retain the morphological and functional characteristics of microglia, the resident immune cells of the central nervous system. Due to the expression of v-raf/v-myc, BV2 cells exhibit an accelerated metabolic and proliferation rate compared to other microglia, making them an ideal tool for various research applications. The expression of nuclear v-myc and cytoplasmic v-RAF oncogene products and the env gp70 antigen on the surface of BV2 cells establishes them as a suitable model for studying macrophages. These cells possess morphological, phenotypical, and functional markers similar to macrophages, expanding their potential applications in immunology research. The role of microglia in neurodegeneration, toxicology, and immunity is an ever-growing field in biomedical research. Traditional studies often rely on animal models, necessitating many animals for experimentation. However, using a microglia-like cell line, such as BV2 cells, offers a promising alternative by providing a continuous and reproducible source of microglia. By using BV2 cells, researchers can accelerate their research programs while minimizing the need for constant cell preparations and animal experimentation. A recent re-evaluation of BV2 cells examined their suitability as a substitute for primary microglia (PM). The response of BV2 cells to lipopolysaccharide (LPS) was compared with that of microglia in both in vitro and in vivo settings. Transcriptome and proteome analyses following LPS stimulation revealed that BV2 cells exhibited a reaction pattern similar to that of PM, although the upregulation of genes was slightly less pronounced on average. Moreover, BV2 cells displayed normal regulation of nitric oxide (NO) production and functional response to interferon-gamma (IFN-gamma), critical parameters for their interaction with T cells and neurons. BV2 cells were also found to stimulate other glial cells effectively, leading to the translocation of NF-kappaB and subsequent production of interleukin-6 (IL-6) in astrocytes. These findings highlight the versatility of BV2 cells and their suitability for studying complex cell-cell interactions. BV2 cells offer a robust and reliable tool for researchers in microglial biology. Their derivation from C57/BL6 murine, coupled with the expression of v-raf/v-myc oncogene products and env gp70 antigen, enables them to retain key characteristics of microglia and macrophages. BV2 cells have proven to be a valid substitute for primary microglia in various experimental settings, facilitating research on neurodegeneration, toxicology, immunity, and cell-cell interactions. By utilizing BV2 cells, scientists can advance their understanding of microglial function and contribute to breakthroughs in biological science.
Organism	Mouse
Tissue	Brain
Synonyms	BV-2

Age	1 week
Gender	Female
Morphology	Morphology microglial
Growth properties	Adherent/suspension

Citation	BV2 (CLS catalog number 305156)
Biosafety level	1

Culture Medium	RPMI 1640
Medium supplements	10% FBS, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
Passaging solution	Accutase
Subculturing	Collect suspension cells in a 15 ml tube and carefully rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes, then centrifuge the cells growing in suspension and the adherent cells together. Carefully resuspend the cells and dispense into new flasks which contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Sterility	Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.
STR profile	M_18-3: 16,17 M_4-2: 20.3 M_6-7: 15 M_3-2: 14 M_19-2: 13 M_7-1: 26.2 M_1-1: 16,17 M_Sex: x M_8-1: 16 M_2-1: 16 M_15-3: 22.3,23.3,24.3 M_6-4: 18 M_11-2: 16 M_1-2: 19 M_17-2: 15 M_12-1: 17 M_5-5: 17 M_X-1: 27 M_13-1: 17 Human D4/D8: -

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Custom projects

Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

BV2

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

Handling

Quality control / Genetic profile / HLA