CLS-439 Cells
Product number:
300150
General information
Description | Established from the primary bladder carcinoma of a 61-year-old male in 1998 by CLS. |
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Organism | Human |
Tissue | Bladder |
Disease | Carcinoma |
Synonyms | CLS439 |
Characteristics
Age | 61 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | CLS-439 (Cytion catalog number 300150) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Handling
Culture Medium | McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 35 hours |
Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent layer in about 3 days |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | The cells must rest for a minimum of 24 hours after thawing at 37 degree Celsius/5% CO2 |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | CLS-439 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 11
D16S539: 10,13
D5S818: 11
D7S820: 10,11
TH01: 7
TPOX: 9,10
vWA: 17
D3S1358: 16
D21S11: 29,31
D18S51: 14
Penta E: 12,16
Penta D: 9,12
D8S1179: 11,13
FGA: 20
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HLA alleles |
A*: 01:01:01, 11:01:01
B*: 08:01:01
C*: 07:01:01
DRB1*: 03:01:01
DQA1*: 05:01:01
DQB1*: 02:01:01
DPB1*: 04:01:01G, 04:02:01G
E: 01:01:01
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