COS-1

€340.00*

Product number: 305005

Description	African green monkey kidney fibroblast cell line COS-1 was first developed in 1981 by J.W.F. Cowell and colleagues. Since COS-1 cells can effectively express exogenous proteins, they are frequently used to produce recombinant proteins and study protein-protein interactions. The constitutively active c-src gene and SV40's large T-antigen boost translation efficiency, which in turn boosts protein expression. The cytopathic effects of viruses and the responses of host cells to viral infection have both been extensively studied in COS-1 cells. Herpes simplex, vesicular stomatitis, and influenza A are just a few of the viruses that they are susceptible to. This makes the COS-1 cells useful for researching how viruses cause host cell responses, pathogenesis, and the creation of antiviral drugs. In general, the COS-1 cell line has advanced our understanding of biological mechanisms. It is a popular model system in molecular and cell biology research due to its capacity to express exogenous proteins with efficiency and its permissiveness?to a variety of viruses.
Organism	Cercopithecus aethiops (Green monkey)
Tissue	Kidney
Synonyms	Cos-1, COS 1, Cos 1, COS1, Cos1, CV-1 in Origin Simian-1

Gender	Male
Morphology	Fibroblast
Growth properties	Adherent

Identifiers / Biosafety / Citation

Citation	COS-1 (CLS catalog number 305005)
Biosafety level	1

Expression / Mutation / Susceptibility

Protein expression	T Antigen, This Is An African Green Monkey Kidney Fibroblast-Like Cell Line Suitable For Transfection By Vectors Requiring Expression Of Sv40 T Antigen. The Cells Are Ebna Negative, Negative For Fc Receptors And Negative For Complement Receptors.

Culture Medium	DMEM
Medium supplements	10% FBS, 4.0 mM L-glutamine, 4.5 g/L glucose, 3.7 g/L NaHCO3
Passaging solution	Accutase
Subculturing	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)
Handling of cryopreserved cultures	The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.
Handling of proliferating cultures	One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.

Quality control / Genetic profile / HLA

Sterility	Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.

Contamination-free cells

To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.

Bulk order

Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.

Authenticated cells

Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).

HLA alleles

HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.