Cell line authentication - Dog

€150.00*

Content: 1 unit
Services
Product number: 900655
Product nr Options Price
900154 Human cells €150.00
900654 Mouse cells €150.00
900655 Dog cells €150.00
900656 Hamster cells €150.00
900657 Rat cells €150.00
Product information "Cell line authentication - Dog"

The authentication of cells which are being used in scientific research projects is an important issue as cross-contamination and misidentification is widespread. About 15-20% of all cell line-based research is working with misidentified cell lines. Therefore, identifying the profile of a cell line by STR analysis is essential for conducting valid and reproducible research. Furthermore, an increasing number of journals require cell line authentication as a prerequisite for manuscript acceptance.

 

Our service includes

  • Cell line authentication
  • Comparison with online data bases
  • Publication-ready analysis report

 

Easy-to-use

  • Please send us the samples in a padded envelope at room temperature. 
  • For gDNA please provide us with ≥ 50 μl of 50ng/μl gDNA in Tris or EDTA (10 mM Tris, 0.1 mM EDTA). 
  • For Cell Pellets, please provide us with 1.0-5.0 million cells as a cell pellet. Please wash twice with PBS and resuspend in 0.5 ml of 70-90% ethanol. 

 

Markers

  • Human cells are typed with the PowerPlex System from Promega using 16 STR marker.
  • Mouse cells are typed with 18 STR markers.
  • Rat cells are typed with 14 STR markers and one sex specific marker.
  • Dog cells are typed with 11 STR markers.
  • Hamster cells are typed with 10 STR markers.

 

Please note that non-European customers may have to pay for customs clearance and veterinary inspection.

 

Results

  • You will get the results within 2 weeks per email. The results include the comparison of the data with the Cellosaurus database. The cell line will be classified as authenticated or misidentified.

 

An exemplary analysis report for the human cell line Raji is shown below. 

 

Summary Table of the STR Profile

Locus

Chromosomal Location

Core STR Marker

Customer Sample Typed Alleles

Database Alleles

D3S1358

Chr03

 

15/16

15/16

TH01

Chr11

Yes

6/7

6/7

D21S11

Chr21

 

28/31

28/31

D18S51

Chr18

 

17

17

Penta_E

Chr15

 

5/13

5/13

D5S818

Chr05

Yes

10/13

10/13

D13S317

Chr13

Yes

13

13

D7S820

Chr07

Yes

10

10

D16S539

Chr16

Yes

8/11

8/11

CSF1PO

Chr05

Yes

10/12

10/12

Penta_D

Chr21

 

3.2/9

3.2/9

AMEL

X/Y

Yes

X/Y

X/Y

vWA

Chr12

Yes

16/19

16/19

D8S1179

Chr08

 

14/15

14/15

TPOX

Chr2

Yes

8/13

8/13

FGA

Chr04

 

19/27

19/27

 

 Electropherogram

Conclusion

According to our analysis of the submitted sample there is no detectable contamination with human origin. The analyzed data of the submitted sample match 100 % to the DNA profile of the cell line Raji (ATCC® CCL-86™) and 100 % over all 15 autosomal STRs to the DNA profile of Raji (Cellosaurus, RRID:CVCL_0511).

Glossary

Short Tandem Repeats (STRs)

Short tandem repeats (STRs) consist of a DNA motif of 2-13 bases that are repeated up to several hundred times. The number of repeats in a STR is highly variable among individuals, resulting in fragment length differences if amplified using PCR. These differences in fragment lengths at different loci are used for profiling the cell lines.

Stutter Peaks

Stutter peaks are small peaks which occur immediately before or after the true peak. Stutter peaks are commonly caused by a slippage of the polymerase during the PCR amplification.

Detection of Cell Line Mixtures

Contamination of one cell line by one or several other cell lines can be detected down to a frequency of the contaminating cell line of 10%. Typically, cell line mixtures will result in STR profiles including three or more peaks for single or multiple loci. If CLS notices a possible contamination of a cell line, we will comment the finding in the conclusion part of the analysis.

Peak height ratio

Peak height ratio <25 % (to the highest peak within a STR) is mentioned in the summary table (comments). Peak height ratios <25% do not necessarily influence the behavior or characteristics of the cell line. A small peak height may be due to reduced amplification efficiency, for example resulting from a mutation in the primer site. The reason for the difference in peak heights observed, however, would need some in depth analysis of the test item.

 

 

References "Cell line authentication - Dog"
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.