Colo-205

€340.00*

Content: 1 cryovial
Product number: 300380
Product information "Colo-205"

Human Colon Adenocarcinoma cell line COLO-205

 

Organism:

Homo sapiens (human)

Ethnicity:

Caucasian

Age:

70 years old

Gender:

Male

Tissue:

Colon (metastatic site: ascites)

Morphology:

Epithelial

Cell type:

Colorectal adenocarcinoma; Dukes' type D

Growth Properties:

Cells grow loosely attached and in suspension

Description:

The cells are CSAp negative (CSAp-).The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.

 

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Required products

Freeze medium CM-ACF, serum free
- Ready-to-use and easy-to-use- Free of serum and animal-derived components- Suitable for long-term cryopreservation- Tested with more than 400 cell lines- Stable for more than 6 monthsCM-ACF is a cryopreservation medium enabling the long-time cryopreservation of human and animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, and consistent results with respect to cell viability following the thawing procedure are at hand.

Variants from €39.00*
€59.00*
Freeze Medium CM-1
- Ready-to-use and easy-to-use- Suitable for long-term cryopreservation- Tested with more than 400 cell lines- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €29.00*
€39.00*
Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

€43.95*
FBS
- Fetal Bovine Serum- Cell culture grade- EU-compliant- Origin: South AmericaWe recommend sterile filtration through 0.22 µm filter. 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*

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Colo-205 growing
Human Colon Adenocarcinoma cell line Colo-205  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:70 years old Gender:Male Tissue:Colon (metastatic site: ascites) Morphology:Epithelial Cell type:Colorectal adenocarcinoma; Dukes' type D Growth Properties:Cells grow loosely attached and in suspension Description:The cells are CSAp negative (CSAp-).The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.  

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Colo-205 gDNA
Genomic DNA isolated from Colo-205 cellsHuman Colon Adenocarcinoma cells Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times.

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Colo-205 cell pellet
Snap frozen cell pellet isolated from COLO-205 cellsHuman Colon Adenocarcinoma cell line Applications:Isolation of RNAIsolation of gDNAProduction of lysatesFixation of the pellet and histochemistry Storage:Cell pellets should be kept on ice or at 4°C at all times.Do not allow to stand at ambient or higher temperatures.Long-term storage at -80°C is recommended.Several freeze-thaw cycles must be avoided. The pellets are shipped on Dry Ice.  

€75.00*