DMEM High Glucose with 10% FBS


Content: 500 milliliter
Product nr Options Price Additional information
820300 with 10% FBS €46.00
820300a basic €18.00
Product number: 820300
Product information "DMEM High Glucose with 10% FBS"

Dulbecco's Modified Eagle Medium (DMEM): An Essential Tool for Cell Culture

Culturing cells is crucial in biological research, as it allows scientists to study cellular behavior, growth, and development. Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium that has proven effective in supporting the growth of a variety of mammalian cells, including primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

What is DMEM and its Composition?

 DMEM is a modified form of the original Eagle's Minimal Essential Medium, developed by Harry Eagle and published in 1959 in Science. The modification includes an increased concentration of amino acids and vitamins, making it a more nutritious medium for cell growth. DMEM uses a sodium bicarbonate buffer system, which helps maintain a physiological pH in a 5–10% CO2 environment.

Various DMEM formulations are available, including those with higher glucose levels, with or without sodium pyruvate, and L-glutamine/GlutaMAX supplementation. The standard DMEM formulation contains four mM L-glutamine, 4500 mg/L glucose, one mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It is important to note that DMEM commonly requires fetal bovine serum (FBS) supplementation for optimal growth of cells.

Choosing the Right DMEM for Your Experiment

In conclusion, Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium that provides essential nutrients to support the growth of a variety of mammalian cells. With a range of formulations and options for customization, DMEM is an indispensable tool for cell culture experiments.

Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.