DU4475 Cells
Product number:
300371
General information
Organism | Human |
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Tissue | Breast |
Disease | Breast carcinoma |
Metastatic site | Skin |
Applications | 3D cell culture, Immuno-oncology |
Synonyms | Du4475, DU-4475, Du-4475, DU 4475, Du 4475, Duke University 4475 |
Characteristics
Age | 62 years |
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Gender | Female |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Clusters in Suspension |
Identifiers / Biosafety / Citation
Citation | DU4475 (Cytion catalog number 300371) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | AK-1, 1, ES-D, 1, G6PD, B, GLO-I, 2, Me-2, 2, PGM1, 1-2, PGM3, 1 |
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Tumorigenic | Yes, in nude mice |
Viruses | EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Karyotype | human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93xxxx, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 - sideline with -20, -20, +del(7)(p11) - gain of 1q and loss of 6q typical in breast carcinoma - resembles published karyotype |
Handling
Culture Medium | RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3 (Cytion article number 820702a) |
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Medium supplements | Supplement the medium with 10% heat-inactivated FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | DU4475 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 9,12
D13S317: 11,14
D16S539: 11,12
D5S818: 11
D7S820: 9,10
TH01: 6,8
TPOX: 8
vWA: 17
D3S1358: 14,16
D21S11: 29,31.2
D18S51: 14,16
Penta E: 7,13
Penta D: 13,14
D8S1179: 10,13
FGA: 22,25
D6S1043: 11
D2S1338: 20,25
D12S391: 18.3,25
D19S433: 14
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