E11 growing culture

€1,060.00*

Content: 1 cryovial
Product variants
Product number: 440494
Product nr Options Price
400494 cryopreserved culture €800.00
400494CP cell pellet - 1 million cells €75.00
400494CP5 cell pellet - 5 million cells €300.00
400494GD5 genomic DNA - 5 microgram €95.00
440494 growing culture €1,060.00
Product information "E11 growing culture"

Murine Kidney podocyte cell line E11

 

Organism:

Mus musculus (mouse)

Stage:

Adult

Strain:

Immorto-Mouse H-2kb-tsA58

Tissue:

Kidney

Cell type:

Podocyte

Growth Properties:

Monolayer, adherent

Description:

The E11 cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes.

 

 

Disclaimer

This cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.

 

Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Required products

Freeze medium CM-ACF, serum free
- Ready-to-use and easy-to-use
- Free of serum and animal-derived components
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-ACF is a cryopreservation medium enabling the long-time cryopreservation of human and animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, and consistent results with respect to cell viability following the thawing procedure are at hand.

€59.00*
Freeze Medium CM-1
- Ready-to-use and easy-to-use
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €59.00*
€39.00*
RPMI 1640
RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. 

€18.00*
Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

€43.95*
FBS
Our Fetal Bovine Serum (FBS) is triple filtered through 0.1 µm filters. Each lot is tested for sterility and for the ability to support the growth of several different cell lines. Fetal bovine serum is manufactured from fetal bovine blood collected at government registered facilities in South America. 
 




Cell lines cultured using FBS















2106T


VERO


FRTL-5




 
Quality control

Each lot has been tested for sterility and absence of mycoplasma and bacteria. 

 
Maintenance 

Keep frozen at -40°C to -10°C. 
The shelf life is 60 months.
Do not heat to more than 37°C and do not use uncontrollable sources of heat (e.g., microwave appliances). 

 
Disclaimer

This product is for in-vitro use only. Not intended for clinical or diagnostic applications.

 
 
 

 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*