EB3 Cells
General information
Organism | Human |
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Tissue | Bone |
Disease | Burkitt lymphoma |
Metastatic site | Bone |
Applications | 3D cell culture, Immunology |
Synonyms | EB-3, Epstein-Barr-3, GM04679 |
Characteristics
Age | 3 years |
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Gender | Male |
Ethnicity | African |
Morphology | Lymphoblast |
Cell type | B lymphocyte |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | EB3 (Cytion catalog number 300373) |
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Biosafety level | 2 |
Expression / Mutation
Surface antigens | HLA A3, Aw32, Cw2 |
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Isoenzymes | G6PD, A |
Viruses | EBV (EBNA pos) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
TH01: 7
TPOX: 6,9
vWA: 17,19
D3S1358: 15,16
D21S11: 29
D18S51: 15,17
Penta E: 14,16
Penta D: 10,11
D8S1179: 14
FGA: 22
D6S1043: 11,13
D2S1338: 17,22
D12S391: 15
D19S433: 12.2,16.2
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