NCI-H647 Cells
General information
Description | NCI-H647 cells are a human lung carcinoma cell line derived from a patient with large cell carcinoma of the lung. This cell line is part of the NCI (National Cancer Institute) panel of human tumor cell lines used extensively in cancer research, particularly in studies concerning lung cancer biology and therapeutics. The NCI-H647 cell line exhibits characteristics typical of large cell lung carcinoma, including rapid growth and the ability to form tumors when xenografted into immunocompromised mice. These cells are particularly useful for exploring the molecular mechanisms of lung cancer pathogenesis, including signal transduction pathways, genetic mutations involved in cancer progression, and the role of tumor microenvironment factors. NCI-H647 cells are often employed in drug screening studies to evaluate the efficacy and toxicity of chemotherapeutic agents and targeted therapies. Their responsiveness to various anti-cancer compounds helps in understanding the pharmacodynamics and potential resistance mechanisms of lung cancer treatments. This cell line is also used to study the interaction between cancer cells and therapeutic agents, providing insights into the development of more effective and personalized treatment strategies for lung cancer patients. Overall, the NCI-H647 cell line serves as a critical tool in lung cancer research, facilitating advancements in understanding the disease and developing novel therapeutic approaches. |
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Organism | Human |
Tissue | Lung |
Disease | Lung adenosquamous carcinoma |
Metastatic site | Pleural effusion |
Synonyms | NCI-H647, H-647, H647ell, NCIH647 |
Characteristics
Age | 56 years |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NCI-H647 (Cytion catalog number 305130) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:3 to 1:6 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 9,11
D16S539: 9
D5S818: 12
D7S820: 10
TH01: 6,9.3
TPOX: 11
vWA: 17
D3S1358: 17
D21S11: 28,32.2
D18S51: 12,15
Penta E: 7
Penta D: 12,13
D8S1179: 11,13
FGA: 22,24
D6S1043: 18,20
D2S1338: 17,25
D12S391: 23
D19S433: 14
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