HBL-100 cell pellet - 1 million cells

€75.00*

Content: 1 cryovial
Product variants
Product number: 300178CP
Product nr Options Price
300178 cryopreserved culture €340.00
300178CP cell pellet - 1 million cells €75.00
300178CP5 cell pellet - 5 million cells €300.00
300178GD5 genomic DNA - 5 microgram €95.00
330178 growing culture €600.00
Product information "HBL-100 cell pellet - 1 million cells"

Manufacturing method

The cells have been cultured to about 80-90% confluence and harvested according to standard cell culture procedures. Detached cells were rinsed twice in ice-cold PBS, collected by centrifugation, and snap-frozen immediately in liquid nitrogen.

Applications

  • Isolation of gDNA
  • Isolation of RNA
  • Production of lysates
  • Fixation of the pellet and histochemistry

Concentration

2.0 million cells per pellet

Quality control

The cell preparation was free of mycoplasma contamination as determined through PCR. Cell viability was >90% ahead of preparing the aliquots.

Storage

Keep the cell pellet at -196°C for long-term storage. Do not store the pellets at -80°C for a longer period. Always keep the cell pellet on ice during preparations.

Note

Please centrifuge before opening the vial.

Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Required products

McCoy's 5a
This medium was developed originally to culture Novikoff Hepatoma cells and successfully applied in the proliferation of a wide range of human and ral normal and transformed cells. The composition includes increased levels of inositol and glucose and contains glutamine.

Variants from €18.00*
€46.00*
Freeze Medium CM-1
- Ready-to-use and easy-to-use
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €59.00*
€39.00*
Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

€43.95*
FBS
Our Fetal Bovine Serum (FBS) is triple filtered through 0.1 µm filters. Each lot is tested for sterility and for the ability to support the growth of several different cell lines. Fetal bovine serum is manufactured from fetal bovine blood collected at government registered facilities in South America. 
 




Cell lines cultured using FBS















2106T


VERO


FRTL-5




 
Quality control

Each lot has been tested for sterility and absence of mycoplasma and bacteria. 

 
Maintenance 

Keep frozen at -40°C to -10°C. 
The shelf life is 60 months.
Do not heat to more than 37°C and do not use uncontrollable sources of heat (e.g., microwave appliances). 

 
Disclaimer

This product is for in-vitro use only. Not intended for clinical or diagnostic applications.

 
 
 

 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*