HEP3B Cells
Product number:
305141
General information about the Hep-3B cell line
Description | The Hep3B cell line, derived from an 8-year-old child with liver cancer, is a pivotal model in the study of human liver cancer cells and their responses to various therapeutic agents. Hep3B cells contain an integrated hepatitis B virus genome and is integral in the investigation of differential drug responses due to its unique genetic and phenotypic characteristics. The Hep 3B human hepatoma cell line is renowned for its extensive expression of liver-specific proteins such as alpha-fetoprotein (AFP), albumin, and various other markers, making it an invaluable tool in drug metabolism and hepatotoxicity studies. This wide array of expressed proteins allows for a comprehensive assessment of how liver cancer cells interact with and metabolize therapeutic agents. The Hep 3B cell line and its derivative cell lines, such as luciferase-expressing variants, enable the tracking of tumor growth and metastasis in vivo, facilitating the study of liver cancer progression and the efficacy of potential treatments. The Hep3B cell line stands out as a crucial resource for advancing our understanding of liver cancer biology and the development of more effective therapeutic strategies. |
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Organism | Human |
Tissue | Liver |
Disease | Childhood hepatocellular carcinoma |
Synonyms | Hep 3B2_1-7, HEP3B217, Hep 3B2, HEP-3B2, HEP3B2, Hep-3B, HEP-3B, Hep 3B, Hep3B, HEP3B |
Features
Age | 8 years |
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Gender | Male |
Ethnicity | African |
Morphology | Epithelial |
Growth properties | Adherent |
Specifications about the liver cancer cell line Hep3B
Citation | Hep 3B2.1-7 (Cytion catalog number 305141) |
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Biosafety level | 1 |
Genetic profile
Protein expression | Alpha Fetoprotein(Alpha-Fetoprotein), Hepatitis B Surface Antigen(Hbsag), Albumin, Alpha2 Macroglobulin(Alpha-2-Macroglobulin), Alpha1 Antitrypsin(Alpha-1-Antitrypsin), Transferrin,, Alpha1 Antichymotrypsin(Alpha-1-Antichymotrypsin), Haptoglobin, Cerulopl |
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Tumorigenic | Yes |
Culturing methods
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | Hep 3B2.1-7 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality assurance
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
TH01: 6,7
TPOX: 9
vWA: 17
D3S1358: 15
D21S11: 30,31
D18S51: 20
Penta E: 5,16
Penta D: 12,14
D8S1179: 12
FGA: 18
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