HFL1 Cells
General information
Organism | Human |
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Tissue | Lung |
Synonyms | HFL-1, HFL 1, Human fetal lung fibroblast 1, HFL |
Characteristics
Age | Fetus |
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Gender | Male |
Morphology | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HFL1 (Cytion catalog number 305065) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12,12
D7S820: 9,10
TH01: 7,9
TPOX: 6,9
vWA: 17,17
D3S1358: 14,17
D21S11: 27,30
D18S51: 18,19
Penta E: 12,20
Penta D: 2.2,9
D8S1179: 12,14
FGA: 21,22
D6S1043: 11,18
D2S1338: 17,25
D12S391: 20,21
D19S433: 11,13
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