HPAF-II Cells
General information
Description | HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes. |
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Organism | Human |
Tissue | Pancreas |
Disease | Pancreatic ductal adenocarcinoma |
Metastatic site | Ascites |
Synonyms | HPAF II, HPAFII, HPAF-2, HPAF2, HPAF/CD18, CD18/HPAF, HPAF-II/CD18, CD-18, CD18, CD 18 |
Characteristics
Age | 44 years |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HPAF-II (Cytion catalog number 305088) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 26 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:5 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 12
D16S539: 11,13
D5S818: 11,13
D7S820: 10,13
TH01: 9
TPOX: 8
vWA: 17
D3S1358: 14,18
D21S11: 30,31
D18S51: 13
Penta E: 10,13
Penta D: 9,13
D8S1179: 11,12
FGA: 21,24
D6S1043: 12
D2S1338: 16,19
D12S391: 17
D19S433: 12
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