HUVEC












General information
Description | HUVECs, or human umbilical vein endothelial cells, are primary cells commonly used in biomedical research to study vascular biology in vitro. These cells are isolated from the umbilical cord vein and are a model system for studying endothelial cell function. Endothelial cells line the inside of blood vessels and play a critical role in vascular homeostasis, angiogenesis, and various vascular diseases such as atherosclerosis. One of the key advantages of using HUVECs in research is their ability to closely mimic the vasculature and study endothelial cell dynamics in vitro. This makes them an important tool in various research fields, including inflammation, oxidative stress, infection response, and normal and tumor-associated angiogenesis. In cancer research, HUVECs are of particular interest due to the ability of tumors to induce angiogenesis. Solid tumors require a healthy supply of oxygen- and nutrient-rich blood to grow and survive, and angiogenic processes direct the formation of new blood vessels, enhancing the tumor blood supply. Developing therapeutic agents that target tumor angiogenesis is an active area of research, and HUVECs play a significant role in this process. HUVECs are a primary, non-immortalized cell system that is easy to isolate and culture, making them an ideal model for studying vascular endothelium. They can be used in standardized in vitro angiogenesis assays to study normal endothelial cell behavior or evaluate how endothelial cells react to different stimuli or treatments with pro- and anti-angiogenic agents. HUVECs can also be used in tube formation assays to assess the ability of endothelial cells to form tubes. This process occurs after endothelial proliferation and migration to the site of the pro-angiogenic stimulus. To culture HUVECs, it takes about 2-4 hours to attach to the surface with 0.2% gelatin coating. Our HUVEC cells have undergone extensive testing to ensure their quality and viability. We guarantee that each vial contains at least 70% viable cells that have been tested for the presence of mycoplasma, bacteria, yeast, or other fungi. Quality control includes testing for the absence of hepatitis B, hepatitis C, and HIV-1 viruses. Our HUVEC cells are cryopreserved at the end of the primary culture phase in a medium containing 10% DMSO, with each vial containing over 0.5 x 10^6 viable cells. In conclusion, HUVECs are a widely used model system to study vascular biology in vitro. |
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Organism | Human |
Tissue | Umbilical vein |
Applications | Human Umbilical Vein Endothelial Cells (HUVECs) are widely used in various biomedical research areas because they can quickly proliferate and differentiate into different types of endothelial cells, which line blood vessels. HUVECs have many research and drug discovery applications, including wound healing, angiogenesis, tissue engineering, inflammation, oncology, pharmacology, vascular modeling, and transfection. |
Synonyms | Human Umbilical Vein Endothelial Cells |
Characteristics
Ethnicity | Caucasian |
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Morphology | Endothelial |
Cell type | Primary cells |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | HUVEC, pooled (CLS catalog number 300605) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | Cytosplasmic VWF/ Factor VIII > 95% positive by immunofluorescence. Cytoplasmic uptake of Di-I-Ac-LDL > 95% positive by immunofluorescence. Cytoplasmic PECAM1 > 95% positive by immunofluorescence |
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Viruses | Negative for HIV-1, HBV, and HCV |
Handling
Culture Medium | Endothelial Cell Growth Medium |
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Medium supplements | 0,1 mg/ml bFGF, w: 2.0 mM stable Glutamine, w/o: Ribonucleosides, w/o: Deoxyribonucleosides, w: 1.0 mM Sodium pyruvate, w: 2.2g/L NaHCO3 |
Passaging solution | Accutase |
Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | Every 2 to 3 days |
Freeze medium | CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100) |
Handling of cryopreserved cultures | The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section. |
Handling of proliferating cultures | One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring. |
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Benötigte Produkte
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
In biological research, the cryopreservation of mammalian cells is an invaluable tool. Successful preservation of cells is a top priority given that losing a cell line to contamination or improper storage conditions leads to lost time and money, ultimately delaying research results. Once the cells have been transferred from a cell growth medium to a freezing medium, the cells are typically frozen at a regulated rate and stored in liquid nitrogen vapor or at below -130°C in a mechanical deep freezer. The freeze medium CM-1 enables cryopreservation of cells at below -130°C (or in liquid nitrogen), essentially eliminating the need for an additional, costly ultralow freezer and eliminating time-consuming and demanding controlled rate freezing processes. Simply collect the cells, aspirate the growth medium, resuspend in CM-1, transfer to a cryovial, and store the vial at below -130 °C.
Long shelf-life
CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.
Trusted by hundreds of researchers
Our advanced cell freezing medium CM-1 is a market-leading product in Germany and Europe and is distinguished by numerous publications involving hundreds of different cell lines worldwide. We tested it with more than 1000 cell lines from our proprietary cell bank.
Optimized ingredients
CM-1 does contain serum products. Serum-containing cryopreservation mediums optimally protect the cells whilst being frozen and have the advantage of high recovery rates. As CM-1 has been tested with a multitude of cell lines, you can rest assured that your cells always recover well.
Contains FBS, DMSO, glucose, salts
Buffering capacity pH = 7.2 to 7.6
Applications & Validation
The cells preserved in our CM-1 freeze medium can be used for cell counting, viability and cryopreservation, cell culture, mammalian cell culture, gene expression analysis and genotyping, in vitro transcription, and polymerase chain reactions. Each batch's efficacy is evaluated using CHO-K1 cells. Each batch is tested for pH, osmolality, sterility, and endotoxins to ensure high quality.
- A Gentle Alternative to Trypsin
Accutase is a cell detachment solution that is revolutionizing the cell culture industry. It is a mix of proteolytic and collagenolytic enzymes that mimics the action of trypsin and collagenase. Unlike trypsin, Accutase does not contain any mammalian or bacterial components and is much gentler on cells, making it an ideal solution for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. In this blog post, we will explore the benefits and uses of Accutase and how it is changing the game in cell culture.
Advantages of Accutase
Accutase has several advantages over traditional trypsin solutions. Firstly, it can be used whenever gentle and efficient detachment of any adherent cell line is needed, making it a direct replacement for trypsin. Secondly, Accutase works extremely well on embryonic and neuronal stem cells, and it has been shown to maintain the viability of these cells after passaging. Thirdly, Accutase preserves most epitopes for subsequent flow cytometry analysis, making it ideal for cell surface marker analysis.
Additionally, Accutase does not need to be neutralized when passaging adherent cells. The addition of more media after the cells are split dilutes Accutase so it is no longer able to detach cells. This eliminates the need for an inactivation step and saves time for cell culture technicians. Finally, Accutase does not need to be aliquoted, and a bottle is stable in the refrigerator for 2 months.
Applications of Accutase
Accutase is a direct replacement for trypsin solution and can be used for the passaging of cell lines. Additionally, Accutase performs well when detaching cells for the analysis of many cell surface markers using flow cytometry and for cell sorting. Other downstream applications of Accutase treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Composition of Accutase
Accutase contains no mammalian or bacterial components and is a natural enzyme mixture with proteolytic and collagenolytic enzyme activity. It is formulated at a much lower concentration than trypsin and collagenase, making it less toxic and gentler, but just as effective.
Efficiency of Accutase
Accutase has been shown to be efficient in detaching primary and stem cells and maintaining high cell viability compared to animal origin enzymes such as trypsin. 100% of cells are recovered after 10 minutes, and there is no harm in leaving cells in Accutase for up to 45 minutes, thanks to autodigestion of Accutase.
In summary
In conclusion, Accutase is a powerful solution that is changing the game in cell culture. With its gentle nature, efficiency, and versatility, Accutase is the ideal alternative to trypsin. If you are looking for a reliable and efficient solution for cell detachment, Accutase is the solution for you.
Phosphate-buffered saline (PBS) is a versatile buffer solution used in many biological and chemical applications, as well as tissue processing. Our PBS solution is formulated with high-quality ingredients to ensure a constant pH during experiments. The osmolarity and ion concentrations of our PBS solution are matched to those of the human body, making it isotonic and non-toxic to most cells.
Composition of our PBS Solution
Our PBS solution is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions. At a 1X working concentration, it contains 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4. We have chosen this composition based on CSHL protocols and Molecular cloning by Sambrook, which are well-established standards in the research community.
Applications of our PBS Solution
Our PBS solution is ideal for a wide range of applications in biological research. Its isotonic and non-toxic properties make it perfect for substance dilution and cell container rinsing. Our PBS solution with EDTA can also be used to disengage attached and clumped cells. However, it is important to note that divalent metals such as zinc cannot be added to PBS as this may result in precipitation. In such cases, Good's buffers are recommended. Moreover, our PBS solution has been shown to be an acceptable alternative to viral transport medium for the transport and storage of RNA viruses, such as SARS-CoV-2.
Storage of our PBS Solution
Our PBS solution can be stored at room temperature, making it easy to use and access.
To sum up
In summary, our PBS solution is an essential component in many biological and chemical experiments. Its isotonic and non-toxic properties make it suitable for numerous applications, from cell culture to viral transport medium. By choosing our high-quality PBS solution, researchers can optimize their experiments and ensure accurate and reliable results.
Definition of Endothelial Cell Growth Media
An in-vitro cell culture system requires a particular solution called Endothelial Cell Growth Media to sustain the endothelial cells necessary for the experiment. Endothelial cells in the body are exposed to an environment mimicked by the medium, including necessary and non-essential amino acids, vitamins, hormones, growth factors, and trace minerals. This nutrient-dense fluid is critical for preserving the endothelium phenotype and function because it stimulates cell proliferation. Globally, researchers and scientists rely on the CLS Endothelial Cell Growth Medium because it is both effective and simple.
Importance of Endothelial Cell Research
Blood artery creation, blood pressure control, and wound healing are just a few physiological processes in which endothelial cells play a key part. To keep the blood vessels balanced, these cells may react to chemical and mechanical stimuli. In order to fully comprehend the causes of hypertension, atherosclerosis, and thrombosis, research into endothelial cells is crucial. Investigating endothelial cells may also provide light on how to treat these diseases medically. Providing a stable and uniform environment for endothelial cell development and proliferation, the CLS Endothelial Cell Growth Medium has shown to be an indispensable tool in this kind of study.
Quality Assurance
High-quality in-vitro cell culture media is essential for obtaining consistent and repeatable outcomes. Quality control testing is performed on every CLS Endothelial Cell Growth Media batch to guarantee its uniformity and effectiveness. The sterility, lack of mycoplasma and bacteria, and pH levels of each batch are examined. To promote healthy cell development and proliferation, the medium's pH is kept at 7.2 +/
- 0.02, and the temperature is maintained between 20 and 25 degrees Celsius. To ensure the culture is clear of any contamination that might impact the accuracy of the findings, the medium is also checked for microbiological pollutants such as fungus, bacteria, and mycoplasma.
Maintenance and Disclaimer
The expiration date of CLS Endothelial Cell Growth Media is six weeks from the production date. The medium should be kept at a temperature of between +2°C and +8°C, away from light, and never frozen or heated over 37°C to preserve its quality. As a corollary, it is crucial to avoid microwaves and other unregulated heat sources that might compromise the product's integrity. A portion of the medium may be taken out of the container and brought up to room temperature if just a little amount will be utilized. CLS's Endothelial Cell Growth Medium is a tried and true method for cultivating endothelial cells because of the stringent quality controls used in its production.
Notably, the CLS Endothelial Cell Growth Medium is not for clinical or diagnostic use; it is designed primarily for in-vitro usage. Obtaining accurate and reliable findings from the medium requires strict adherence to the manufacturer's guidelines and quality control methods.
Applications of the Medium
The CLS Endothelial Cell Growth Medium has proven useful in several biological studies. As this medium may be used to cultivate endothelial cells, it can be used to simulate endothelial function and malfunction in organ systems in vitro. This is especially useful for studying the blood-brain barrier or tissue-engineered blood arteries. This is essential for understanding disease processes and creating effective treatments.
Endothelial cells, especially HUVEC cells, have been extensively employed in angiogenesis research, wound healing research, and cancer research, thus, the medium has also been modified to promote their proliferation. Accurate and repeatable findings depend on the consistency and high quality of the medium used to develop these cells, and the CLS Endothelial Cell Growth Media meets both of these requirements.



