HuCC-T1 Cells
Product number:
300469
General information
Organism | Human |
---|---|
Tissue | Liver |
Disease | Intrahepatic cholangiocarcinoma |
Metastatic site | Ascites |
Applications | Studies of the mechanism of tumor marker secretion and tumor cell growth in the human cholangiocellular carcinoma |
Synonyms | HuCCT-1, HUCCT-1, HUCC-T1, HUCCT1, HuCCT1 |
Characteristics
Age | 56 years |
---|---|
Gender | Male |
Ethnicity | Japanese |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | HuCC-T1 (Cytion catalog number 300469) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice. |
---|
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Trypsin-EDTA |
Subculturing | Discard the old medium and wash the cells with PBS. Add a freshly prepared 0.025% trypsin/0.02% EDTA solution heated to 37 degrees Celsius and wait until the cells detach, which usually takes about 5 minutes. Neutralize the trypsin by adding fresh medium, then transfer the cell mixture to a tube and centrifuge. After centrifugation, remove the supernatant, resuspend the cell pellet in fresh culture medium, and transfer the suspension to new flasks. Incorporate G418 into the culture medium to achieve a final concentration of 0.5 mg/ml |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | HuCC-T1 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 11,13
D16S539: 11,12
D5S818: 12,13
D7S820: 10,11
TH01: 7,10
TPOX: 8
vWA: 18
D3S1358: 15
D21S11: 31
D18S51: 13
Penta E: 15,18
Penta D: 10
D8S1179: 10
FGA: 20,23
D6S1043: 13
D2S1338: 17,18
D12S391: 18,20
D19S433: 13
|