IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.0 mM Sodium pyruvate, w: 3.0 g/L NaHCO3
Exploring Iscove's Modified Dulbecco's Medium (IMDM): A Comprehensive Guide for Cell Culture Applications
Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. It is a modification of Dulbecco's Modified Eagle Medium (DMEM) and contains selenium and different amino acids and vitamins. In this blog post, we will explore the components of IMDM, its uses, and the differences between IMDM and other cell culture media.
What is IMDM?
IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH.
How is IMDM used?
IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.
IMDM vs. DMEM
IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.
IMDM vs. RPMI
IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
To sum up
In conclusion, IMDM is a complex and enriched growth medium for cell culture well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. It is a modification of DMEM and contains selenium and different amino acids and vitamins. IMDM lacks iron and requires supplementation with FBS, and uses a sodium bicarbonate buffer system. The various modifications of IMDM make it suitable for various cell culture applications. The differences between IMDM and other cell culture media, such as DMEM and RPMI make it essential to choose the appropriate media for specialized cell types and specific applications.
