KYSE-410 Cells
General information
Description | This cell line was established from the poorly differentiated invasive esophageal squamous cell carcinoma from the cervical esophagus of a 51-year-old Japanese male prior to treatment (tumor invasion into the adventitia was obvious). The cells were reported to have an overexpression of heparin binding growth factor (hst-1) and cyclin D. |
---|---|
Organism | Human |
Tissue | Esophagus |
Disease | Esophageal squamous cell carcinoma |
Synonyms | KYSE 410, KYSE410, Kyse410, KYSE0410 |
Characteristics
Age | 51 years |
---|---|
Gender | Male |
Ethnicity | Asian |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | KYSE-410 (Cytion catalog number 305122) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 32 to 45 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1: 4 to 1: 6 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
TH01: 8
TPOX: 8,11
vWA: 16,18
D3S1358: 15,16
D21S11: 30
D18S51: 13,15
Penta E: 8,12
Penta D: 11
D8S1179: 10
FGA: 20
D6S1043: 13,15
D2S1338: 17,19
D12S391: 17,19
D19S433: 13
|