MKN-45 Cells
General information
Description | This cell line was established from a poorly differentiated gastric adenocarcinoma. |
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Organism | Human |
Tissue | Stomach |
Disease | Gastric adenocarcinoma |
Metastatic site | Liver |
Synonyms | MKN 45, MKN45 |
Characteristics
Age | 62 years |
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Gender | Female |
Ethnicity | Japanese |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | MKN-45 (Cytion catalog number 300489) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 20% heat-inactivated FBS |
Passaging solution | Accutase |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 8,11
D16S539: 10
D5S818: 10,11
D7S820: 10,11
TH01: 7
TPOX: 8
vWA: 19
D3S1358: 15,16
D21S11: 31
D18S51: 16
Penta E: 10
Penta D: 10
D8S1179: 13,17
FGA: 19,24
D6S1043: 14
D2S1338: 18
D12S391: 26
D19S433: 14,16.2
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