MML-1

€540.00*

Content: 1 cryovial
Product number: 300288
Product information "MML-1"

Human Skin Melanoma cell line MML-1

 

Organism:

Homo sapiens (human)

Tissue:

Skin

Morphology:

Epithelial

Cell type:

Malignant melanoma

Growth Properties:

Monolayer, adherent

STR-Analysis:

This MML-1 cell line shows a unique profile.

Reference:

Concepción de Inés, Björn Cochlovius, Stefanie Schmidt, Sergey Kipriyanov, Hans-Jürgen Rode, and Melvyn Little. Apoptosis of a Human Melanoma Cell Line Specifically Induced by Membrane-Bound Single-Chain Antibodies1. The Journal of Immunology, 163: 3948-3956, 1999.

 

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Required products

Freeze Medium CM-1
- Ready-to-use and easy-to-use- Suitable for long-term cryopreservation- Tested with more than 400 cell lines- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €29.00*
€39.00*
RPMI 1640
RPMI-1640 represents an excellent cell culture medium for human and animal lymphocytes, either normal or neoplastic. 

€18.00*
Accutase
Accutase Cell Detachment solution is a ready-to-use cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and polymers. Accutase performs exceptionally well in detaching cells for the analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.Accutase does not contain mammalian or bacterial derived products.  

€43.95*
FBS
- Fetal Bovine Serum- Cell culture grade- EU-compliant- Origin: South AmericaWe recommend sterile filtration through 0.22 µm filter. 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*

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MML-1 growing
Human Skin Melanoma cell line MML-1  Organism:Homo sapiens (human) Tissue:Skin Morphology:Epithelial Cell type:Malignant melanoma Growth Properties:Monolayer, adherent STR-Analysis:This MML-1 cell line shows a unique profile. Reference:Concepción de Inés, Björn Cochlovius, Stefanie Schmidt, Sergey Kipriyanov, Hans-Jürgen Rode, and Melvyn Little. Apoptosis of a Human Melanoma Cell Line Specifically Induced by Membrane-Bound Single-Chain Antibodies1. The Journal of Immunology, 163: 3948-3956, 1999.  

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MML-1 gDNA
Genomic DNA isolated from MML-1 cellsHuman Skin Melanoma cells Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times. 

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MML-1 cell pellet
Snap frozen cell pellet isolated from MML-1 cellsHuman Skin Melanoma cell line Applications:Isolation of RNAIsolation of gDNAProduction of lysatesFixation of the pellet and histochemistry Storage:Cell pellets should be kept on ice or at 4°C at all times.Do not allow to stand at ambient or higher temperatures.Long-term storage at -80°C is recommended.Several freeze-thaw cycles must be avoided. The pellets are shipped on Dry Ice.  

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