Mycoplasma long term testing
Mycoplasmas are small, prokaryotic organisms which are a frequent contamination of cell cultures. Mycoplasma are not visible by microscopic analysis. However, the contamination may affect the vitality of cell lines which slows down the proliferation rate and morphology. Approximately 15 -35% of cell cultures are contaminated. Therefore it is essential that all cell stocks are routinely checked for mycoplasmas to secure a mycoplasma-free environment.
We conduct highly sensitive, specific cell-based as well as PCR-based tests for the most common species of mycoplasma, such as M. Hyorhinis, M. Arginini, M.Orale, M.Fermentans and A. laidlawii which represent 98% of tissue culture infections.
The Mycoplasma Long Assay serves to detect mycoplasma during antibiotics-free cell cultivation at 37°C for 14 days via mycoplasma-specific qPCR and a sensitive cell-based test.
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