Human cell lines
Animal cell lines
Media & Reagents
|Description||This cell line was established in 1986 from a 55-year-old female smoker (15 pack years) with non-small cell lung carcinoma. Another cell line, NCI-BL1395 (BL1395), is a lymphoblastoid line from the same patient.|
|Synonyms||NCI-H1395, H-1395, NCIH1395|
|Citation||H1395 (CLS catalog number 305118)|
|Culture Medium||RPMI 1640|
|Medium supplements||10% FBS, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3|
|Subculturing||Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.|
|Split ratio||1: 3 to 1: 4|
|Fluid renewal||2 to 3 times per week|
|Freeze medium||CM-1 (CLS catalog number 800100) or CM-ACF (CLS catalog number 806100)|
|Handling of cryopreserved cultures||The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150 degree Celsius after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 degree Celsius water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the subculture section.|
|Handling of proliferating cultures||One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 degree Celsius for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 degree Celsius for a minimum of 24 hours.|
|Sterility||Mycoplasma contamination was excluded through PCR-based and luminescence-based mycoplasma assays. Bacterial or fungal contaminations are detected through daily visual cell monitoring.|
Penta E: 7
Penta D: 12
This Modal is powered by moori Foundation
Functional cookies are absolutely necessary for the functionality of the web shop. These cookies assign a unique random ID to your browser so that your unhindered shopping experience can be guaranteed over several page views.
Tracking cookies help the shop operator to collect and evaluate information about the behaviour of users on their website.
Service cookies are used to provide the user with additional offers (e.g. live chats) on the website. Information obtained via these service cookies may also be processed for site analysis.
These cookies are used for statistics and shop performance metrics.
Marketing cookies are used to display advertisements on the website in a targeted and individualized manner across multiple page views and browser sessions.
Request an offer