NCI-H23 Cells
General information
Description | This line was established from lung cancer tissue obtained from a 59-year-old black male lung adenocarcinoma patient prior to therapy. The cells carry the mutation of K-ras 12 and a mutation in the codon 246 (ATC ?ATG, isoleucine -> methionine) of the p53 gene. The cells express C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs. The cell line has a heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR). NCI-H23 exhibits a 20-fold higher level of c-myc DNA amplification without detectable c-myc RNA amplification. The cells stain positive for keratins 5+8 and 18 and vimentin but negative for neurofilament. The cells are L-dopa decarboxylase-negative. The cells can form colonies in soft agarose with an efficiency of 9.7%. |
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Organism | Human |
Tissue | Lung |
Disease | Lung adenocarcinoma |
Synonyms | NCI-H23, NCI.H23, NCI H23 , H-23, NCIH23 |
Characteristics
Age | 51 years |
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Gender | Male |
Ethnicity | African |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NCI-H23 (Cytion catalog number 305044) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | myc+, src+, abl+, erb+, ras+, sis - |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 38 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1: 3 to 1: 4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 9,10
TH01: 6
TPOX: 8,9
vWA: 16,17
D3S1358: 15
D21S11: 30
D18S51: 14
Penta E: 7,17
Penta D: 8
D8S1179: 15
FGA: 24
D6S1043: 12
D2S1338: 18,23
D12S391: 15,17
D19S433: 12,14
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