NCI-H508 Cells
Product number:
305107
General information
Description | This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. |
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Organism | Human |
Tissue | Large intestine, Cecum |
Disease | Cecum adenocarcinoma |
Metastatic site | Abdominal Wall |
Synonyms | NCI H508, NCIH508, H-508, NCI-H508 |
Characteristics
Age | 55 years |
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Gender | Male |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Mixed: floating aggregates of round cells with some attached cells |
Identifiers / Biosafety / Citation
Citation | NCI-H508 (Cytion catalog number 305107) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | carcinoembryonic antigen (CEA) 1709 ng/mL per 106 cells per 10 days |
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Antigen expression | Blood type A, Rh+, CA19-9 antigen |
Tumorigenic | Yes |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 32 hours |
Subculturing | Remove and discard culture medium. These cells grow as a mixture of floating and adherent cells. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 1.0 to 2.0 mL of Accutase solution to flask and observe cells under a microscope until the cell layer is dispersed (usually within 2 to 3 minutes). Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37 degree Celsius. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures | NCI-H508 cells are shipped in a deep-frozen state on dry ice. Upon receipt, confirm that the vial remains frozen. For storage, place the cryovial immediately at temperatures below -150 degrees. If you plan to culture the cells immediately, swiftly thaw the vial by shaking it in a 37 degrees water bath with clean water and an antimicrobial agent for 40-60 seconds. Remove the vial once a small ice clump persists, ensuring it remains cold. Proceed with all subsequent steps under aseptic conditions. In a sterile flow hood, disinfect the cryovial with 70% ethanol. Then, gently open the vial and transfer the cell suspension into a 15 ml centrifuge tube pre-filled with 8 ml of room temperature culture medium. Gently mix the cells. For cell separation, centrifuge at 300 x g for 3 minutes and dispose of the supernatant. Skipping centrifugation is optional, although any residual freezing medium should be removed after 24 hours. Resuspend the pellet gently in 10 ml of fresh culture medium and divide between two T25 culture flasks. Follow the subculture protocol for subsequent steps. |
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is rigorously excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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