NCI-H520 Cells
General information
Description | The cell line was established in 1982 from a sample of a lung mass taken by A.F. Gazdar from a patient with squamous cell carcimoma of the lung. A greatly reduced level of p53 mRNA is expressed by this cell line compared to normal lung tissue. The cells exhibit no gross structural DNA abnormalities. The cells stain positive for keratin and vimentin but negative for neurofilament triplet protein. The cells can form colonies in soft agar with/without serum. |
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Organism | Human |
Tissue | Lung |
Disease | Lung squamous cell carcinoma |
Synonyms | NCI-H520, H-520, NCI-HUT-520, NCIH520 |
Characteristics
Gender | Male |
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Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | NCI-H520 (Cytion catalog number 305063) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in nude mice inoculated subcutaneously with 1?10^7 cells (Tumors developed within 21 days at 100% frequency (5/5)). |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 32 to 60 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1: 3 to 1: 4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 10,11
D16S539: 13
D5S818: 12,13
D7S820: 8,12
TH01: 10
TPOX: 8
vWA: 18,19
D3S1358: 16
D21S11: 30
D18S51: 17
Penta E: 5,14
Penta D: 13
D8S1179: 14,16,17
FGA: 22
D1S1656: 14,16.3
D6S1043: 12,18
D2S1338: 18,23
D12S391: 21
D19S433: 13,14
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