Nalm-6 genomic DNA - 5 microgram
Product information "Nalm-6 genomic DNA - 5 microgram"
Manufacturing method
The genomic DNA (gDNA) is isolated by cell lysis followed by the addition of Proteinase K and RNAse A and purification on columns. The conditions have been chosen to allow for PCR or other enzymatic reactions in the downstream process. The fragment length of the purified DNA is up to 50kb.
Applications
- PCR / RT-PCR / qPCR
- Next-generation sequencing (NGS)
- Single nucleotide polymorphism (SNP) analysis
- Genomic analysis
- Gene expression studies
- Southern Blot
Concentration
50-100 ng/µl in TE Buffer (10 mM Tris-CL, 0.5 mM EDTA, pH 8.0). If you require a specific concentration, please contact us.
Quality control
- The quality and purity of the DNA are tested with a spectrophotometer. Absorption at A260/280 is between 1.8 and 2.0. Contamination with RNA, proteoglycans, and polysaccharides is excluded.
- We can quantify the double-stranded DNA (dsDNA) content free of charge, please contact us for more information.
- Please contact us for further information if you require DNA from HLA-typed cell lines.
- Store at 4°C for frequent use or at -20°C for occasional use.
- For prolonged storage (> 6 months) we recommend -80°C.
- Avoid more than three freeze/thaw cycles.
Storage
- Store at 4°C for frequent use or at -20°C for occasional use.
- For prolonged storage (> 6 months) we recommend -80°C.
- Avoid more than three freeze/thaw cycles.
Note
Please centrifuge before opening the vial.
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Custom projects
Besides genomic DNA, RNA, cell pellets, and cell lysates, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.