PK-15 growing culture

€600.00*

Content: 1 cryovial
Product variants
Product number: 667426
Product nr Options Price
607426 cryopreserved culture €340.00
607426CP cell pellet - 1 million cells €75.00
607426CP5 cell pellet - 5 million cells €300.00
607426GD5 genomic DNA - 5 microgram €95.00
667426 growing culture €600.00
Product information "PK-15 growing culture"

Pig Kidney cell line PK-15

 

Organism:

Sus scrofa (pig)

Synonym(s):

Swine

Age/Stage:

Adult

Tissue:

Kidney, normal

Morphology:

Epithelial

Growth Properties:

Monolayer, adherent

Description:

The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining.

 

 

According to Az. 45244.0179, November 2018, the ZKBS has classified the cell line PK-15 into Risk group 2 due to infection with the porcine polytropic endogene retrovirus (polytropic PERV), Gen TSV § 5,1. A transfer into humans resulting in insertion mutagenesis cannot be not excluded.

 

 

 

 

Properties "PK-15 growing culture"
Product variants: cryopreserved culture, growing culture, genomic DNA - 5 microgram, cell pellet - 1 million cells
Contamination-free cells
To identify mycoplasma contaminations we perform PCR-based and luminescence-based mycoplasma assays. We further determine any bacterial or fungal contamination through our standardized manufacturing processes.
Bulk order
Besides genomic DNA, RNA, cell pellets, and cell lysates in bulk, we can offer large quantities of assay-ready cells, plated cells in multiple formats, and frozen or growing cells. Contact us to receive a quote.
Authenticated cells
Each manufactured batch of cell lines* is authenticated via STR analysis. Contact us if you require a publication-ready STR report for your cells (*human, hamster, mouse, rat, and dog cells).
HLA alleles
HLA characterization is available from more than 200 cell lines. HLA class I -A, B, C, and Class II HLA-DPA1, -DPB1, -DQA1, DQB1, and DRB1 alleles were obtained by next-generation sequencing methodologies (NGS) for class I and class II alleles.

Required products

Freeze Medium CM-1
- Ready-to-use and easy-to-use
- Suitable for long-term cryopreservation
- Tested with more than 400 cell lines
- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Variants from €59.00*
€39.00*
EMEM
EMEM (Minimum Essential Medium Eagle) represents a cell culture medium that is well suited for the propagation of a wide variety of cell lines. Cell growth is supported due to the addition of L-glutamine. This culture medium is recommended for adherent cell types. 

€18.00*
FBS
Our Fetal Bovine Serum (FBS) is triple filtered through 0.1 µm filters. Each lot is tested for sterility and for the ability to support the growth of several different cell lines. Fetal bovine serum is manufactured from fetal bovine blood collected at government registered facilities in South America. 
 




Cell lines cultured using FBS















2106T


VERO


FRTL-5




 
Quality control

Each lot has been tested for sterility and absence of mycoplasma and bacteria. 

 
Maintenance 

Keep frozen at -40°C to -10°C. 
The shelf life is 60 months.
Do not heat to more than 37°C and do not use uncontrollable sources of heat (e.g., microwave appliances). 

 
Disclaimer

This product is for in-vitro use only. Not intended for clinical or diagnostic applications.

 
 
 

 

Variants from €25.20*
€219.00*
PBS
Phosphate buffered solutionSterile, applicable in cell culture 

€7.00*