- Ready-to-use and easy-to-use- Suitable for long-term cryopreservation- Tested with more than 400 cell lines- Stable for more than 6 monthsCM-1 represents a cryopreservation medium developed by CLS enabling the long-time cryopreservation of Human and Animal cells and cell lines at -196°C. For more than 15 years it has been used successfully at CLS, therefore consistant results with respect to cell viability following the thawing procedure are at hand. 

Human Lung (primary tumor) carcinoma cell line 2106T  Depositor:M. Meister, Thoraxklinik Heidelberg Organism:Homo sapiens (human) Ethnicity:Caucasian Age:51 years Gender:Male Tissue:Lung primary tumor Morphology:Mid-size and polygonal with membrane protrusions, prominent nucleoli and intercellular bridges Celltype:Squamous Cell Carcinoma Growth Properties:monolayer, adherent Description:The 2106T cell line has been established from pulmonary squamous cell carcinoma (SCC) of a patient Dr. Sandra Gottschling and Dr. Michael Meister in 2009. 2106LN cell line was isolated from a lymph node metastasis of the same patient.   DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.

Cell pellet manufactured from 2106THuman Lung (primary tumor) carcinoma cells Applications:Isolation of RNAIsolation of gDNAProduction of lysatesFixation of the pellet and histochemistry Storage:Cell pellets should be kept on ice or at 4°C at all times.Do not allow to stand at ambient or higher temperatures.Long-term storage at -80°C is recommended.Several freeze-thaw cycles must be avoided. 

Genomic DNA isolated from 2106T Human Lung (primary tumor) carcinoma cell line Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C.

Human Lung (primary tumor) carcinoma cell line 2106T  Depositor:M. Meister, Thoraxklinik Heidelberg Organism:Homo sapiens (human) Ethnicity:Caucasian Age:51 years Gender:Male Tissue:Lung primary tumor Morphology:Mid-size and polygonal with membrane protrusions, prominent nucleoli and intercellular bridges Celltype:Squamous Cell Carcinoma Growth Properties:monolayer, adherent Description:The 2106T cell line has been established from pulmonary squamous cell carcinoma (SCC) of a patient by Dr. Sandra Gottschling and Dr. Michael Meister in 2009. 2106LN cell line was isolated from a lymph node metastasis of the same patient.   Unique combination of a primary tumor- (2106T) and lymph node metastasis-derived (2106LN) SCC cell lines.  DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information. 

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Murine Embryo Fibroblast cell line 3T3-L1  Synonym:3T3 Swiss Albino Organism:Mus musculus (mouse) Stage:Embryo Tissue:Embryo Morphology:Fibroblast Growth Properties:Monolayer, adherent Description:The 3T3 cell line was established from 17 to 19 days old mouse embryos.  The cells are contact inhibited. Species:Mouse origin was confirmed by Real-Time-PCR.  

Snap frozen cell pellet isolated from 3T3-L1 (3T3 Swiss Albino) cellsMurine Embryo Fibroblast cell line  Applications:Isolation of RNAIsolation of gDNAProduction of lysatesFixation of the pellet and histochemistry Storage:Cell pellets should be kept on ice or at 4°C at all times.Do not allow to stand at ambient or higher temperatures.Long-term storage at -80°C is recommended.Several freeze-thaw cycles must be avoided. The pellets are shipped on Dry Ice. 

Genomic DNA isolated from 3T3-L1 (3T3 Swiss Albino) cells Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times.  

Murine Embryo Fibroblast Zelllinie 3T3-L1  Synonym: 3T3 Swiss Albino Organism:Mus musculus (mouse) Stage:Embryo Tissue:Embryo Morphology:Fibroblast Growth Properties:Monolayer, adherent Description:The 3T3 cell line was established from 17 to 19 days old mouse embryos.  The cells are contact inhibited Species:Mouse origin was confirmed by Real-Time-PCR.  

Murine Embryo Fibroblast cell line 3T6-Swiss albino  Organism:Mus musculus (mouse) Stage:Embryo Morphology:Fibroblast Growth Properties:Monolayer, adherent Description:The 3T6 cell line was established from 17 to 19 days old mouse embryos. Species:Mouse origin was confirmed by Real-Time PCR.   

Genomic DNA isolated from 3T6 Swiss Albino cells Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times.  

Murine Embryo Fibroblast cell line 3T6-Swiss albino  Organism:Mus musculus (mouse) Stage:Embryo Morphology:Fibroblast Growth Properties:Monolayer, adherent Description:The 3T6 cell line was established from 17 to 19 days old mouse embryos. Species:Mouse origin was confirmed by Real-Time PCR.  

Human Urinary bladder carcinoma cell line 5637  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:68 years of age Gender:Male Tissue:Bladder (urinary) Morphology:Epithelial Cell type:Carcinoma Growth Properties:Monolayer, adherent Description:The 5637 cell line has been established from the primary bladder carcinoma (grade II) of a patient by Dr. G. Cannon in 1974.  

Snap frozen cell pellet isolated from 5637 cellsHuman Urinary bladder carcinoma cell line Applications:Isolation of RNAIsolation of gDNAProduction of lysatesFixation of the pellet and histochemistry Storage:Cell pellets should be kept on ice or at 4°C at all times.Do not allow to stand at ambient or higher temperatures.Long-term storage at -80°C is recommended.Several freeze-thaw cycles must be avoided. The pellets are shipped on Dry Ice.

Genomic DNA isolated from 5637 cellsHuman Urinary bladder carcinoma cells Applications: PCR, RT-PCRSouthern BlotSNP AnalysisGenomic DNA library constructionStudies on gene expression Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times. 

Human Urinary bladder carcinoma cell line 5637  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:68 years of age Gender:Male Tissue:Bladder (urinary) Morphology:Epithelial Cell type:Carcinoma Growth Properties:Monolayer, adherent Description:The 5637 cell line has been established from the primary bladder carcinoma (grade II) of a patient by Dr. G. Cannon in 1974.  

Origin and General Characteristics Organism                                             Human Ethnicity                                              Caucasian Age                                                      63 Gender                                                 Female Tissue                                                  Kidney Disease                                                Renal cell carcinoma Morphology                                        Epithelial-like Growth properties                               Adherent Depositor                                            H. Löhrke Cellosaurus accession ID                   CVCL_1050 Wikidata                                             Q54604830 NCBI_TaxID                                      9606 Description                                         This line was derived from a primary clear cell adenocarcinoma. The cells are globular with                                                                      indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and                                                                          desmosomes. They can be cultured in soft agar. References                                          References are available in the product page at www.cls.shop                 Culture Conditions and Handling   Culture medium                     RPMI 1640, 10% FBS Supplements 2 mM L-glutamine Doubling time 35 hours Subculturing Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Split ratio A ratio of 1:4 to 1:12 is recommended Seeding density 3 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days. Fluid renewal Every 2 to 3 days Freeze medium CM-1 (CLS catalog number 800150) Freeze medium After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. Sterility Plasmotest: negative; Mycoplasma specific PCR: negative           Special Features of the Cell Line Tumorigenic Yes, in immuno-suppressed hamsters and nude mice Viruses SMRV: Negative, as confirmed by Real-Time PCR Karyotype Hypodiploid; modal number = 45; a large submetacentric chromosome was present in all cells.     DNA profile (STR)   Amelogenin CSF1PO X,X 11,12 D18S51 Penta E 14,17 7,18   D13S317 10,14 Penta D 12,16   D16S539 9,13 D8S1179 12,16   D5S818 12 FGA 20,22   D7S820 10,11 D1S1656 n.a.   TH01 6,9.3 D6S1043 n.a.   TPOX 8,11 D2S1338 n.a.   vWA 18 D12S391 n.a.   D3S1358 16 D19S433 n.a.   D21S11 28,30               HLA-typing A* n.a., n.a. DQB1* n.a., n.a.   B* n.a., n.a. DPA1* n.a., n.a.   C* n.a., n.a. DPB1* n.a., n.a.   DRB1* n.a., n.a. E n.a., n.a.   DRB3* n.a., n.a. G n.a., n.a.   DRB4* n.a., n.a. F n.a., n.a.   DRB5* n.a., n.a. MR-1 n.a., n.a.   DQA1* n.a., n.a.                         Recommendations for handling of cells following delivery Cryopreserved cells The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150°C after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 °C water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the Subculture section. Proliferating cultures   The cell culture flasks, 2xT25, come filled with cell culture medium. Collect the entire medium in 2 x 50 ml centrifuge tubes. Carefully add 5 ml of cell culture medium to each of the two T25 cell culture flasks. Control the cell morphology and confluency under the microscope. Incubate at 37 °C for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to 1 x T25 cell culture. Incubate at 37 °C for a minimum of 24 hours.     Legal & Documentation Safety precautions If the cryovial is planned to be stored in liquid nitrogen and to be thawed in the future, special safety precautions should be followed. Protective gloves and clothing should be used and a facemask or safety goggles must be worn when transferring frozen samples into or removing from the liquid nitrogen tank. The removal of a cryovial from liquid nitrogen may result in the explosion of the frozen vial creating flying fragments. Warranty CLS warrants for a high cell viability and culture performance only if the products are stored and cultured according to the information described in this product sheet. Certificate of Analysis The certificate of analysis can be requested by email at info@cls.shop for each batch. Please indicate the lot number of your product in the email. Disclaimer This product is intended for laboratory research use only. It is strictly prohibited in any case to use the cells, their progeny, unmodified derivatives and modifications in human or animal subjects for therapeutic, diagnostic or prophylactic purposes. All regulations as described in the Material Transfer Agreement or Supply Agreement concluded with the order apply.