Human keratinocyte cell line HaCaT
Depositor:DKFZ, Heidelberg
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Age:62 years
Gender:Male
Tissue:Skin
Cell type:Keratinocyte
Growth Properties:Monolayer, adherent
Description:In vitro spontaneously transformed keratinocytes from histologically normal skin.
Generated by:Dr. N. Fusenig, Dr. P. Boukamp
DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.
€800.00*
This cell line is licensed to CLS by a university or institute. The sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchaser. Please contact us for more information.
Exosomes were isolated from HaCaT human keratinocytes growing as adherent monolayer. The exosomes are stored in PBS containing 25 mM Hepes, pH=7.2. The protein concentration was tested using the BCA assay. Protein concentration was adjusted to 25µg protein / 100µl buffer. Particle size: 141.3 ± 15.2 nm Particle concentration: 0,9E+11/ml Exosomal marker: The presence of the biomarkers CD9, CD63 und CD81 were verified by ELISA. Storage: The exosomal particle should be stored at -80°C. Please avoid repeated thaw/freeze cycles. Shipping conditions: The exosomes come frozen on dry ice. Applications:Exosomes are optimal carrier for various biomarker such as proteins, RNA and lipids.
Genomic DNA isolated from HaCaT cellsHuman keratinocytes Applications: PCR, RT-PCR; Southern Blot; SNP Analysis; Genomic DNA library construction; Studies on gene expression. Storage:Store at 4°C for frequent use.Store at -20°C for occasional use.For prolonged storage (>6 months) we recommend -70°C. Do not freeze-thaw more than three times.
Human keratinocyte cell line HaCaT
Depositor:DKFZ, Heidelberg
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Age:62 years
Gender:Male
Tissue:Skin
Cell type:Keratinocyte
Growth Properties:Monolayer, adherent
Description:In vitro spontaneously transformed keratinocytes from histologically normal skin.
Generated by:Dr. N. Fusenig, Dr. P. Boukamp
DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.
€1,050.00*
This cell line is licensed to CLS by a university or institute. The sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchaser. Please contact us for more information.
Human keratinocyte cell line HaCaT-ras A5Benign variant clone
Depositor:DKFZ, Heidelberg
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Age:62 years
Gender:Male
Tissue:Skin
Cell type:Keratinocyte
Growth Properties:Monolayer, adherent
Description:Spontaneously immortalized human skin keratinocytes (HaCaT) were transfected with the c-Ha-ras (EJ) oncogene, clones were selected on the basis of G418 resistance, as described in detail in Boukamp et al. 1990. HaCaT-ras A5 clonal cells form highly differentiated, cystic benign tumors upon injection into Balb/c-nu/nu mice.
Generated by:Dr. N. Fusenig, Dr. P. Boukamp
DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.
€800.00*
This cell line is licensed to CLS by a university or institute. The sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchaser. Please contact us for more information.
Human keratinocyte cell line HaCaT-ras II-4Malignant variant clone
Depositor:DKFZ, Heidelberg
Organism:Homo sapiens (human)
Ethnicity:Caucasian
Age:62 years
Gender:Male
Tissue:Skin
Cell type:Keratinocyte
Growth Properties:Monolayer, adherent
Description:Spontaneously immortalized human skin keratinocytes (HaCaT) were transfected with the c-Ha-ras (EJ) oncogene, clones were selected on the basis of G418 resistance, as described in detail in Boukamp et al. 1990. HaCaT-ras II-4 clonal cells form highly differentiated, locally invasive squamous cell carcinomas upon injection into Balb/c-nu/nu mice.
Generated by:Dr. N. Fusenig, Dr. P. Boukamp
DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.
€800.00*
This cell line is licensed to CLS by a university or institute. The sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchaser. Please contact us for more information.
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