Human Lung (primary tumor) carcinoma cell line 2106T  Depositor:M. Meister, Thoraxklinik Heidelberg Organism:Homo sapiens (human) Ethnicity:Caucasian Age:51 years Gender:Male Tissue:Lung primary tumor Morphology:Mid-size and polygonal with membrane protrusions, prominent nucleoli and intercellular bridges Celltype:Squamous Cell Carcinoma Growth Properties:monolayer, adherent Description:The 2106T cell line has been established from pulmonary squamous cell carcinoma (SCC) of a patient Dr. Sandra Gottschling and Dr. Michael Meister in 2009. 2106LN cell line was isolated from a lymph node metastasis of the same patient.   DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information.

Human Lung (primary tumor) carcinoma cell line 2106T  Depositor:M. Meister, Thoraxklinik Heidelberg Organism:Homo sapiens (human) Ethnicity:Caucasian Age:51 years Gender:Male Tissue:Lung primary tumor Morphology:Mid-size and polygonal with membrane protrusions, prominent nucleoli and intercellular bridges Celltype:Squamous Cell Carcinoma Growth Properties:monolayer, adherent Description:The 2106T cell line has been established from pulmonary squamous cell carcinoma (SCC) of a patient by Dr. Sandra Gottschling and Dr. Michael Meister in 2009. 2106LN cell line was isolated from a lymph node metastasis of the same patient.   Unique combination of a primary tumor- (2106T) and lymph node metastasis-derived (2106LN) SCC cell lines.  DisclaimerThis cell line is licensed to CLS by a partnering university or research institute. The cell line may be subject to individual terms and licensing fees as required by the licensor. Please contact us for further information. 

Human Lung Carcinoma cell line A-427  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:52 years Gender:Male Tissue:Lung Morphology:Epithelial Cell type:Carcinoma Growth Properties:Monolayer, adherent Description:The A-427 cell line was established by D.J. Giard in 1973.  

Human Lung Carcinoma cell line A-427  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:52 years Gender:Male Tissue:Lung Morphology:Epithelial Cell type:Carcinoma Growth Properties:Monolayer, adherent Description:The A-427 cell line was established by D.J. Giard in 1973.   

Human Lung Carcinoma cell line A549 (A-549)   Organism:Homo sapiens (human) Ethnicity:Caucasian Age:58 years Gender:Male Tissue:Lung Morphology:Epithelial Cell type:Carcinoma Growth Properties:Monolayer, adherent Description:The cells are positive for keratin by immunoperoxidase staining.   

Human Lung Carcinoma cell line A-549 (A549)  Organism:Homo sapiens (human) Ethnicity:Caucasian Age/Stage:58 years Gender:Male Tissue:Lung Morphology:Epithelial Celltype:Carcinoma Growth Properties:Monolayer, adherent Description:The cells are positive for keratin by immunoperoxidase staining.  

Human Lung Carcinoma cell line Calu-1  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:47 years old Gender:Male Tissue:Lung (from metastatic site: pleura);  grade III Morphology:Epithelial Cell type:Epidermoid carcinoma Growth Properties:Monolayer, adherent Description:This cell line shows ultrastructural features such as numerous microvilli, prominent RER,     lysosomes, lipid inclusions. Virus particles are not observed.  Cells contain the K-ras oncogene.  

Human Lung Carcinoma cell line Calu-1  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:47 years old Gender:Male Tissue:Lung (from metastatic site: pleura);  grade III Morphology:Epithelial Cell type:Epidermoid carcinoma Growth Properties:Monolayer, adherent Description:This cell line shows ultrastructural features such as numerous microvilli, prominent RER,     lysosomes, lipid inclusions. Virus particles are not observed.  Cells contain the K-ras oncogene.  

Human Lung Adenocarcinoma cell line Calu-6  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:61 years old Gender:Female Tissue:Anaplastic carcinoma; unknown, probably lung Morphology:Epithelial Growth Properties:Monolayer, adherent Description:Established in 1975 by Fogh et al.  

Human Lung Adenocarcinoma cell line Calu-6  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:61 years old Gender:Female Tissue:Anaplastic carcinoma; unknown, probably lung Morphology:Epithelial Growth Properties:Monolayer, adherent Description:Established in 1975 by Fogh et al.  

DesignationCLS-54 Biosafety level 1CitationCLS-54 (CLS catalog number 300227)  General characteristicsOrganism                               HumanEthnicity                                CaucasianAge                                         65Gender                                   MaleTissue                                    LungDisease                                  AdenocarcinomaMorphology                           Epithelial-likeGrowth properties                AdherentDepositor                               H. LöhrkeCellosaurus accession ID    CVCL_5728NCBI_TaxID                           9606Description                           Established in vitro from the primary lung carcinoma of a 65 year-old man in 1998 by CLS.  Culture conditionsCulture medium                     RPMI 1640, 10% FBSMedium Supplements           2 mM L-glutamineSubculturing                           Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.Split ratio                                A ratio of 1:2 to 1:6 is recommendedSeeding density                     1 x 10^4 cells/cm^2 will yield in a confluent layer in about 4 daysFluid renewal                          Every 3 to 5 daysFreeze medium                      CM-ACF (CLS catalog number 800650)Freezing recovery                  After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.Sterility                                   Plasmotest: negative; Mycoplasma specific PCR: negative         Special featuresTumorigenic                          Yes, in nude miceViruses                                   SMRV: Negative, as confirmed by Real-Time PCRDNA ProfileAmelogenin                           X,XCSF1PO                                  12D13S317                                  11D16S539                                 12,13D5S818                                   13D7S820                                  10,11TH01                                      6,9.3TPOX                                      8,9vWA                                        14,17D3S1358                                 18D21S11                                    30,31.2DD18S51                                 11,17,18Penta E                                  12,15Penta D                                  9D8S1179                                 11FGA                                        20 Handling of cells following deliveryCryopreserved cells              The cells come deep-frozen shipped on dry ice. Please make sure that the vial is still frozen. If immediate culturing is not intended, the cryovial must be stored below -150°C after arrival. If immediate culturing is intended, please follow the below instructions: Quickly thaw by rapid agitation in a 37 °C water bath within 40-60 seconds. The water bath should have clean water containing an antimicrobial agent. As soon as the sample has thawed, remove the cryovial from the water bath. A small ice clump should still remain and the vial should still be cold. From now on, all operations should be carried out under aseptic conditions. Transfer the cryovial to a sterile flow cabinet and wipe with 70% alcohol. Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). Resuspend the cells carefully. Centrifuge at 300 x g for 3 min and discard the supernatant. The centrifugation step may be omitted, but in this case the remains of the freeze medium have to be removed 24 hours later. Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. All further steps are described in the Subculture section.Proliferating cultures            One or two cell culture flasks come filled with cell culture medium. Collect the entire medium in 1 or 2 x 50 ml centrifuge tubes, respectively. Carefully add 5 ml of cell culture medium to each T25 cell culture flask. Control the cell morphology and confluency under the microscope. Incubate at 37 °C for a minimum of 24 hours. Spin down the collected medium at 300 x g for 3 minutes to collect the cells which may have detached during transit. If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. Incubate at 37 °C for a minimum of 24 hours. DocumentationSafety precautions                If the cryovial is planned to be stored in liquid nitrogen and to be thawed in the future, special safety precautions should be followed. Protective gloves and clothing should be used and a facemask or safety goggles must be worn when transferring frozen samples into or removing from the liquid nitrogen tank. The removal of a cryovial from liquid nitrogen may result in the explosion of the frozen vial creating flying fragments.Warranty                                CLS warrants for a high cell viability and culture performance only if the                                                    products are stored and cultured according to the information described                                                  in this product sheet.Certificate of Analysis          The certificate of analysis can be requested on the website or via email                                                     at info@cls.shop. Please indicate the lot number of your product in the                                                       email.Disclaimer                              This product is intended for laboratory research use only. It is strictly                                                         prohibited to use the products in human or animal subjects for                                                                     therapeutic, diagnostic or prophylactic purposes.

Human Lung Carcinoma cell line DMS-79  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:65 years old Tissue:Lung Cell type:Small cell lung carcinoma Growth Properties:Aggregates in suspension Description:The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy. The cells express HLA class I and class II antigens.  

Human Lung Carcinoma cell line DMS-79  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:65 years old Tissue:Lung Cell type:Small cell lung carcinoma Growth Properties:Aggregates in suspension Description:The line was established from cells in the pleural fluid of a patient with small cell carcinoma of the lung. The patient had previously been treated with cytoxan, vincristine, methotrexate and radiation therapy. The cells express HLA class I and class II antigens.  

Human Lung, Metastasis Histiocytoma cell line GCT  Organism:Homo sapiens (human) Age:29 years Gender:Male Tissue:Histiocytoma, fibrous; from metastatic site: lung Growth Properties:Monolayer, adherent Description:The line produces CSA for human granulocyte precursors and EEA for erythroid precursor. Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.  

Human Lung, Metastasis Histiocytoma cell line GCT  Organism:Homo sapiens (human) Age:29 years Gender:Male Tissue:Histiocytoma, fibrous; from metastatic site: lung Growth Properties:Monolayer, adherent Description:The line produces CSA for human granulocyte precursors and EEA for erythroid precursor. Medium conditioned by this line can be used as a source of prostaglandin E and plasminogen activator.  

Human Lung Mesothelioma cell line H-MESO-1  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:65 years Gender:Male Tissue:Lung Morphology:Epithelial Celltype:Mesothelioma Growth Properties:Monolayer, adherent  

Human Lung Mesothelioma cell line H-Meso-1  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:65 years Gender:Male Tissue:Lung Morphology:Epithelial Celltype:Mesothelioma Growth Properties:Monolayer, adherent  

Human Lung Mesothelioma cell line H-Meso-1A  Organism:Homo sapiens (human) Ethnicity:Caucasian Age:65 years Gender:Male Tissue:Mesothelioma Morphology:Epithelial Growth Properties:Monolayer, adherent Description:H-Meso-1A is a subclone of H-Meso-1. DNA anlysis has shown that both cell lines have almost identical STR-profiles except for THO1.