Cell pellets and their applications
Human and animal cells can be harvested after a certain cultivation time and stored safely at -80° C as cell pellets for downstream applications. Cell pellets are a good opportunity to provide reliable and robust control material in different research areas.
Each pellet comprises 1 or 5 million cells and has several potential uses in areas including polymerase chain reaction (PCR), western blotting, building a genomic DNA library, and gene expression profiling as well as for immunohistochemistry (IHC) and situ hybridization (ISH).
Preparation & Quality control
The cells have been cultured to about 80-90% confluence and harvested according to standard cell culture procedures. Detached cells were rinsed twice in ice-cold PBS, collected by centrifugation, and snap-frozen immediately in liquid nitrogen. To ensure high and consistent quality, the cell preparation was determined to be free of mycoplasma and a high cell viability over 90% ahead of preparing the aliquots was measured. Each lot cell lines is further authenticated via STR analysis.
Shipping & Storage
Keep the cell pellet below -80 °C for long-term storage. Always keep the cell pellet on ice during preparations. Cell pellets are shipped on dry ice.