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RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3, w/o: Phenol red
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3, w/o: Phenol red
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The distinctive formulation of this RPMI includes a concentration of 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and is free from phenol red.
Quality Control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3
RPMI 1640, w: 4.5 g/L Glucose, w: 2 mM L-Glutamine, w: 10 mM HEPES, w: 1 mM Sodium pyruvate, w: 1.5 g/L NaHCO3
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
This RPMI 1640 medium contains 4.5 grams per liter of glucose.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA
EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA
One of the most widely used synthetic cell culture media is Minimum Essential Medium Eagle (MEM). Developed by Harry Eagle, this medium was first introduced in 1959 and has since become a popular choice for various cell types grown in monolayers and adherent cell lines.
This EMEM medium consists of 2 mM L-Glutamine, 1.5 g/L NaHCO3, EBSS, 1 mM Sodium pyruvate, and NEAA.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, L-glutamine, sodium pyruvate, and sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, storing the medium at two °C to 8°C in the dark when not in use is recommended.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation. 
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3
Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3
Introducing Ham's F-12K (Kaighn's) Medium, a specialized modification of Ham's F-12 medium designed to meet the unique requirements of biological research. This advanced medium offers distinct advantages, enhancing the cultivation of primary human hepatocytes, as well as rat and chicken liver cells, particularly in reduced serum conditions.

Ham's F-12K (Kaighn's) Medium is carefully formulated to optimize cell culture conditions. It features an enriched composition, providing elevated levels of essential components such as amino acids and sodium pyruvate, as well as additional elements including putrescine, thymidine, hypoxanthine, and zinc. These additions enable researchers to supplement the medium with minimal serum or defined components for specific cell types, facilitating precise experimental conditions.

Notably, Ham's F-12K (Kaighn's) Medium does not contain proteins or growth factors. Consequently, supplementation with growth factors and Fetal Bovine Serum (FBS) is often necessary, allowing researchers to tailor the medium to the requirements of their specific cell lines. For optimal performance, the concentration of FBS must be carefully optimized for each cell line, ensuring optimal growth and functionality.

To maintain physiological pH, Ham's F-12K (Kaighn's) Medium employs a sodium bicarbonate buffer system (2.5 g/L), necessitating a controlled 5-10% CO2 environment during cultivation. This ensures the medium's pH remains within the ideal range for cell growth and viability.

Quality control

pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium but the basic medium is 8 weeks from the date of manufacture.

€18.00*
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3
RPMI 1640 Medium, also known as RPMI medium, is a highly versatile cell culture medium widely utilized in biological research to cultivate various mammalian cells. Developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at the renowned Roswell Park Comprehensive Cancer Center, this medium derived its name from its origin at the Roswell Park Memorial Institute (RPMI).
Initially designed to support the growth of human leukemic cells in both suspension and monolayer cultures, RPMI 1640 Medium has evolved through modifications by researchers and commercial suppliers to become suitable for a diverse range of mammalian cells. It is exceptionally compatible with cell lines such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium stands apart from other cell culture media due to its unique composition. It contains a substantial amount of phosphate, amino acids, and vitamins. Notably, it encompasses biotin, vitamin B12, and PABA, absent in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. Moreover, RPMI 1640 Medium exhibits significantly elevated concentrations of vitamins inositol and choline. However, it does not contain proteins, lipids, or growth factors. Consequently, supplementation with 10% Fetal Bovine Serum (FBS) is commonly required to provide optimal conditions for cell growth.
The buffering system of RPMI 1640 Medium relies on sodium bicarbonate (2.0 g/L) and necessitates a 5-10% CO2 environment to maintain a physiologically appropriate pH. The inclusion of the reducing agent glutathione further distinguishes this medium from others.
The unique composition of this RPMI formulation comprises 2.1 mM of stable Glutamine, 2.0 grams per liter of NaHCO3, and phenol red.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS
EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS
One of the most widely used synthetic cell culture media is Minimum Essential Medium Eagle (MEM). Developed by Harry Eagle, this medium was first introduced in 1959 and has since become a popular choice for various cell types grown in monolayers and adherent cell lines.
This EMEM medium is formulated with 2 mM L-Glutamine, 2.2 g/L NaHCO3, and EBSS.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, L-glutamine, sodium pyruvate, and sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, storing the medium at two °C to 8°C in the dark when not in use is recommended.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation. 
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

Composition




Ingredients


Concentration (mg/L)




Inorganic salts


 




CaCl2 ∙ 2 H2O


264.92




KCl


400.00




Mg SO4


97.67




NaCl


6800.00




NaHCO3


2200.00




NaH2PO4 ∙ H2O


140.00




Amino acids


 




L-Arginine ∙ HCl


126.00




L-Cystine


24.00




L-Histidine ∙ HCl ∙ H2O


42.00




L-Isoleucine


52.00




L-Leucine


52.00




L-Lysine ∙ HCl


72.50




L-Methionine


15.00




L-Phenylalanine


32.00




L-Threonine


48.00




L-Tryptophane


10.00




L-Tyrosine


36.00




L-Valine


46.00




Vitamins


 




Choline chloride


1.00




Folic acid


1.00




myo-Inositol


2.00




Thiamine  ∙ H2O


1.00




Riboflavin


0.10




D-Calcium Pantothenate


1.00




Nicotinamide


1.00




Pyridoxal  ∙ H2O


1.00




Other components


 




D(+)-Glucose


1000.00




L-Glutamine


292.00




Phenol Red


10.00

€18.00*
DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3
DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3
DMEM:Ham's F12 is a widely recognized and extensively utilized basal medium in cell culture for biological research. It serves as a fundamental source of nutrients for the growth of various mammalian cell lines, particularly when supplemented with Fetal Bovine Serum (FBS).

This unique formulation combines Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 (Ham's Nutrient Mixture F-12) in a precise 1:1 ratio. The addition of L-glutamine further enhances its composition.

DMEM, derived from Eagle's Minimal Essential Medium (EMEM), offers an increased concentration of amino acids and vitamins compared to its predecessor. In contrast, Ham's F-12 is based on Ham's F-10 medium, providing a complementary set of essential components.

To support optimal cell growth, it is common practice to supplement DMEM:Ham's F12 with FBS at a typical concentration of 5-10%. This addition is necessary as the medium lacks growth hormones, lipids, and proteins crucial for cellular development.

DMEM:Ham's F12 incorporates a pH buffer system and is often supplemented with phenol red, a pH indicator. Cultured cells in DMEM:Ham's F12, or any medium utilizing the bicarbonate buffer system, require a controlled CO2 environment of 5-10% to maintain appropriate pH levels. Phenol red enables monitoring of pH changes from 6.2 (yellow) to 8.2 (red).
Quality Control

pH = 7.2 +/
- 0.02 at 20-25°C.  
Each lot has been tested for sterility and absence of mycoplasma and bacteria. 

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.  
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).  
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature. 
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture. 

€18.00*
McCoy’s 5A medium (modified), w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3
McCoy’s 5A medium (modified), w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3
McCoy's 5A Medium is a highly recommended and specialized medium designed to facilitate the growth and replication of viruses in primary cell cultures. It has gained significant recognition for its exceptional performance in various biological research applications.
One prominent application of McCoy's 5A Medium is its utilization in the culturing of human colon carcinoma cell lines. Specifically, it has been employed in the study of the leucine-rich repeat-containing G-protein-coupled receptor (LGR5) and its role in the metastasis of colon cancer. This medium has been effectively employed in the cultivation of several colon carcinoma cell lines, including HCT116, RKO, FET, CBS, HCT116b, and TENN, enabling researchers to delve deeper into the mechanisms underlying colon cancer metastasis.
In addition to its application in cancer research, McCoy's 5A Medium has proven to be indispensable in the study of osteoblasts. Researchers investigating the ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media have utilized this medium to culture osteoblasts. This application has facilitated a better understanding of the interactions between osteoblasts and calcium-deficient hydroxyapatite, contributing to advancements in the field of bone research.
Notably, McCoy's 5A Medium was meticulously formulated by modifying the amino acids found in Basal Medium Eagle to provide optimal support for liver tumor cells. This enriched formulation enables its suitability for a diverse range of established cell lines, as well as primary cells, further enhancing its versatility and applicability in various research settings.
Moreover, McCoy's 5A Medium extends its biochemical and physiological effects beyond liver tumor cells. It has been successfully employed to support growth in primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenal, lung, spleen, rat embryo, and other cell types. This wide range of applications attests to the broad utility of McCoy's 5A Medium in supporting the growth and maintenance of various cell types for comprehensive biological research.
Formulation
This McCoy's 5A medium (modified) contains 3.0 g/L of Glucose, stable Glutamine, 2.0 mM of Sodium pyruvate, and 2.2 g/L of NaHCO3.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS
Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS
Medium 199 is a versatile and widely used cell culture medium specially formulated for the cultivation of primary explants. This comprehensive medium combines essential vitamins, amino acids, and other factors to provide a fully defined nutrient source for a variety of cell types. It is particularly suitable for non-transformed cells, making it an invaluable tool for biological research.
Medium 199 offers a range of applications in the field. It can effectively maintain the cumulus-oocyte complex (COC) and support the in vitro maturation of oocytes. Additionally, it is employed in the rinsing of aspiration lines during ovum collection from German Holstein cows. Moreover, Medium 199 serves as an excellent medium for the culture of cardiac endothelial cells derived from rats. These applications demonstrate the versatility and adaptability of Medium 199 to various experimental needs.
History
The development of Medium 199 in the 1950s marked a significant advancement in tissue culture media. Prior to its introduction, many culture media relied on animal-derived products and tissue extracts. However, Morgan and colleagues revolutionized the field by formulating a completely defined nutritional source for cell cultures. Through their experiments involving different combinations of vitamins, amino acids, and other factors, they discovered the exceptional growth-promoting properties of Medium 199.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.0 mM Sodium pyruvate, w: 3.0 g/L NaHCO3
IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.0 mM Sodium pyruvate, w: 3.0 g/L NaHCO3
Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH.
IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.
IMDM vs. DMEM
IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.
IMDM vs. RPMI
IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
Ham's F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
Ham's F12 Medium, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.176 g/L NaHCO3
Ham's F-12 Medium, also known as Ham's F-12 Nutrient Mix, is a widely used basal medium designed specifically for cell culture. It has been extensively employed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Additionally, Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA).
One of its notable advantages is the ability to support cell growth without the need for serum supplementation. This eliminates potential interference caused by serum components, ensuring consistent and reliable experimental results. By providing a serum-free culture environment, Ham's F-12 Medium offers researchers greater control over their investigations.
Another key feature of Ham's F-12 Medium is its suitability for single-cell plating. This makes it an excellent choice for a variety of cell lines, including CHO cells, lung cells, and mouse L cells. The medium's optimized nutrient composition facilitates efficient attachment and growth of individual cells, enabling the establishment of homogeneous cell cultures with improved reproducibility.
Moreover, Ham's F-12 Medium has gained recognition as the preferred medium for the Clonal Toxicity Assay (CTA). This assay plays a critical role in assessing the cytotoxic effects of substances on cells. By utilizing Ham's F-12 Medium in the CTA, researchers can accurately evaluate the impact of various compounds or treatments on individual cells, providing valuable insights into toxicological profiles.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

 

€18.00*
DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a highly versatile and widely utilized basal medium designed to support the growth of a diverse range of mammalian cells in biological research. It serves as an ideal medium for culturing primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, as well as popular cell lines like HeLa, 293, Cos-7, and PC-12.
What sets DMEM apart from other media is its unique composition. It contains an impressive fourfold increase in amino acid and vitamin concentration compared to the original Eagle's Minimal Essential Medium. Initially developed with low glucose (1 g/L) and sodium pyruvate, DMEM is frequently employed with higher glucose levels, either with or without sodium pyruvate. Notably, DMEM does not contain proteins, lipids, or growth factors, necessitating supplementation. To achieve optimal growth, a common approach is to supplement DMEM with 10% Fetal Bovine Serum (FBS). Additionally, DMEM employs a sodium bicarbonate buffer system (3.7 g/L), requiring a 5-10% CO2 environment to maintain a physiological pH.
Dulbecco's Modified Eagle Medium (DMEM) is highly regarded among defined media for cell and tissue culture, catering to the growth needs of various adherent cell phenotypes. It surpasses the original Eagle's Medium, developed in the 1950s for cultivating chicken cells, through the enhanced supplementary formulation known as Dulbecco's modification. This modification significantly elevates the content of select amino acids and vitamins up to fourfold compared to the original medium.
In the field of cell culture, DMEM plays a vital role as a growth medium for different cell types, including primary cells, stem cells, and transformed cells. Researchers also employ the modified version of DMEM for a wide array of research applications, such as drug discovery, tissue engineering, and the study of cell signaling pathways.
Quality control

pH = 7.2 +/
- 0.02 at 20-25°C. 
Each lot has been tested for sterility and absence of mycoplasma and bacteria.

Maintenance  

Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. 
Do not heat the medium to more than 37° C or use uncontrollable sources of heat (e.g., microwave appliances). 
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

Composition




Ingredients


Concentration (mg/L)




Inorganic salts


 




CaCl2 anhydrous


200.00




Fe(NO3)3 ∙ 9H2O


0.10




KCl


400.00




MgSO4 (anhydrous)


97.66




NaCl


6,400.00




NaHCO3


1,500.00




NaH2PO4 ∙ H2O


108.69




Amino acids


 




L-Arginine ∙ HCl


84.00




L-Cystine ∙ 2HCl


62.58




L-Glutamine


584.00




Glycine


30.00




L-Histidine ∙ HCl ∙ H2O


42.00




L-Isoleucine


104.80




L-Leucine


104.80




L-Lysine ∙ HCl


146.20




L-Methionine


30.00




L-Phenylalanine


66.00




L-Serine


42.00




L-Threonine


95.20




L-Tryptophane


16.00




L-Tyrosine ∙ Na


103.79




L-Valine


93.60




Vitamins


 




D-Calcium pantothenate


4.00




Choline chloride


4.00




Folic acid


4.00




myo-Inositol


7.00




Nicotinamide


4.00




Pyridoxine ∙ HCl


4.00




Riboflavin


0.40




Thiamine ∙ HCl


4.00




Other components


 




D (+)-Glucose anhydrous


4,500.00




Sodium pyruvate


110.00




Phenol Red


15.00

€18.00*


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