It is suitable for a range of applications, such as culturing primary cells, stem cells, and immortalized cell lines. RPMI 1640 is also used in various immunological assays, such as ELISAs and co-cultures. The RPMI-1640 medium has been validated extensively over the years to ensure its reliability and consistency. It is recommended by experts in the field of cell biology for use in experiments requiring robust growth and proliferation of mammalian cell types. Its composition makes RPMI 1640 an ideal choice for many complex research applications.
References
1) Merskey, R., & Pappenheimer, A. M. (1963). A New Medium For The Cultivation Of Leukemia Cells And Other Tissue-Culture Cells. Proceedings of the Society for Experimental Biology and Medicine, 112(2), 845–849.
2) RPMI-1640: a protein-free medium optimized for growth characteristics of human hematopoietic cells. (1984). In Vitro Cellular & Developmental Biology, 20(4), 467–475.
When it comes to cell culture, finding a suitable medium is crucial to ensure optimal growth and health of cells. One of the most widely used synthetic cell culture media is Minimum Essential Medium Eagle (MEM). Developed by Harry Eagle, this medium was first introduced in 1959 and has since become a popular choice for a variety of cell types grown in monolayers and attached cell lines.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, two mM L-glutamine, one mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, it's recommended to store the medium at two °C to 8°C in the dark when not in use.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells, and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation.
EMEM 2X contains:
Twice the amount of MEM.
Four times the vitamins and amino acids of the original MEM.
Earle's salts which allows for the culturing of an even wider variety of more nutritionally fastidious cells.
In conclusion, Minimum Essential Medium Eagle (EMEM) is a widely used synthetic cell culture medium that has been instrumental in the cultivation of various cell types. With its higher concentration of amino acids and accurate approximation of the protein composition of cultured mammalian cells, EMEM is a popular choice for maintaining cells in tissue culture. While it shares some similarities with other cell culture media, EMEM's unique formulation and properties make it a valuable addition to any cell culture laboratory.
It is suitable for a range of applications, such as culturing primary cells, stem cells, and immortalized cell lines. RPMI 1640 is also used in various immunological assays, such as ELISAs and co-cultures. The RPMI-1640 medium has been validated extensively over the years to ensure its reliability and consistency. It is recommended by experts in the field of cell biology for use in experiments requiring robust growth and proliferation of mammalian cell types. Its composition makes RPMI 1640 an ideal choice for many complex research applications.
References
1) Merskey, R., & Pappenheimer, A. M. (1963). A New Medium For The Cultivation Of Leukemia Cells And Other Tissue-Culture Cells. Proceedings of the Society for Experimental Biology and Medicine, 112(2), 845–849.
2) RPMI-1640: a protein-free medium optimized for growth characteristics of human hematopoietic cells. (1984). In Vitro Cellular & Developmental Biology, 20(4), 467–475.
When it comes to cell culture, finding a suitable medium is crucial to ensure optimal growth and health of cells. One of the most widely used synthetic cell culture media is Minimum Essential Medium Eagle (MEM). Developed by Harry Eagle, this medium was first introduced in 1959 and has since become a popular choice for a variety of cell types grown in monolayers and attached cell lines.
What's in EMEM?
EMEM is a modified version of Eagle's minimum essential medium, containing Earle's Balanced Salt Solution, non-essential amino acids, two mM L-glutamine, one mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It's important to note that this reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in the air. To maintain its effectiveness, it's recommended to store the medium at two °C to 8°C in the dark when not in use.
What is EMEM used for?
Eagle's minimal essential medium (EMEM) is a cell culture medium that can maintain cells in tissue culture. The medium contains higher concentrations of amino acids, allowing for a more accurate approximation of the protein composition of cultured mammalian cells. EMEM may be used to cultivate various cells, including fibroblasts, human liver cancer cell line (HepG2) cells, and human fetal brain progenitor-derived astrocyte cells (PDA). It is typically used in the presence of fetal bovine serum (FBS), calf, or horse sera.
How is EMEM different from other cell culture media?
While EMEM and Dulbecco's modified Eagle's medium (DMEM) share some similarities, they also differ. Both media lack protein and contain the amino acids, salts, glucose, and vitamins required to provide a cell with energy and maintain it in tissue culture. However, the DMEM formulation is modified to contain up to four times more vitamins and amino acids and two to four times more glucose than EMEM. It's worth noting that EMEM is also different from the original MEM formulation.
EMEM 2X contains:
Twice the amount of MEM.
Four times the vitamins and amino acids of the original MEM.
Earle's salts which allows for the culturing of an even wider variety of more nutritionally fastidious cells.
In conclusion, Minimum Essential Medium Eagle (EMEM) is a widely used synthetic cell culture medium that has been instrumental in the cultivation of various cell types. With its higher concentration of amino acids and accurate approximation of the protein composition of cultured mammalian cells, EMEM is a popular choice for maintaining cells in tissue culture. While it shares some similarities with other cell culture media, EMEM's unique formulation and properties make it a valuable addition to any cell culture laboratory.
Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. It is a modification of Dulbecco's Modified Eagle Medium (DMEM) and contains selenium and different amino acids and vitamins. In this blog post, we will explore the components of IMDM, its uses, and the differences between IMDM and other cell culture media.
What is IMDM?
IMDM is a modification of DMEM containing selenium and has additional amino acids, vitamins, and inorganic salts compared to DMEM. It lacks iron and requires supplementation with Fetal Bovine Serum (FBS). IMDM uses a sodium bicarbonate buffer system and requires a 5-10% CO2 environment to maintain physiological pH.
How is IMDM used?
IMDM is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. The various modifications of IMDM available for a range of cell culture applications can be found using the media selector tool. Liquid media provide essential nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the initially published formula or modifications necessary to the consistent performance and stability of the culture medium.
IMDM vs. DMEM
IMDM contains potassium nitrate instead of ferric nitrate and HEPES and sodium pyruvate. The additional components in IMDM make it more suitable for specialized cell types and specific applications than DMEM.
IMDM vs. RPMI
IMDM and RPMI have different formulations that may be relevant for PMA/ionomycin stimulation. One significant difference is the concentration of Ca2+. While RPMI contains 0.42 mM Ca2+, IMDM contains 1.49 mM.
To sum up
In conclusion, IMDM is a complex and enriched growth medium for cell culture well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. It is a modification of DMEM and contains selenium and different amino acids and vitamins. IMDM lacks iron and requires supplementation with FBS, and uses a sodium bicarbonate buffer system. The various modifications of IMDM make it suitable for various cell culture applications. The differences between IMDM and other cell culture media, such as DMEM and RPMI make it essential to choose the appropriate media for specialized cell types and specific applications.
Culturing cells is crucial in biological research, as it allows scientists to study cellular behavior, growth, and development. Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium that has proven effective in supporting the growth of a variety of mammalian cells, including primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is DMEM and its Composition?
DMEM is a modified form of the original Eagle's Minimal Essential Medium, developed by Harry Eagle and published in 1959 in Science. The modification includes an increased concentration of amino acids and vitamins, making it a more nutritious medium for cell growth. DMEM uses a sodium bicarbonate buffer system, which helps maintain a physiological pH in a 5–10% CO2 environment.
Various DMEM formulations are available, including those with higher glucose levels, with or without sodium pyruvate, and L-glutamine/GlutaMAX supplementation. The standard DMEM formulation contains four mM L-glutamine, 4500 mg/L glucose, one mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It is important to note that DMEM commonly requires fetal bovine serum (FBS) supplementation for optimal growth of cells.
Choosing the Right DMEM for Your Experiment
In conclusion, Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium that provides essential nutrients to support the growth of a variety of mammalian cells. With a range of formulations and options for customization, DMEM is an indispensable tool for cell culture experiments.