This special RPMI-based medium is adapted for NFS-60 and TF-1 cells as it is supplemented with L-glutamine, 10% KMG-2, 10% FBS and GM-CSF.

Glioblastoma is a highly malignant central nervous system tumor, representing over 60% of all brain tumors in adults. GBM is a severe medical condition that has been associated with poor prognosis. Scientists and researchers continuously study gliomas' biology to find effective treatments for patients. Glioblastoma Cell Growth Medium is a serum-free and xeno-free medium designed to support the growth of glioblastoma cell lines, including fibroblast cells.
Discover our Glioblastoma Cell Growth Medium
Glioblastoma Cell Growth Medium is a high-quality and ready-to-use medium that is specially formulated to support the growth and maintenance of glioblastoma cell lines. The medium is serum-free and xeno-free, which ensures the absence of animal-derived components. This medium is the successor to GBM-MG and has been adapted to support the growth of fibroblast cells, particularly human Gingival Fibroblasts.
Cells tested with our Glioblastoma Cell Growth Medium

NCH612
NCH421K
NCH690

Composition of the medium
Glioblastoma Cell Growth Medium contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, and trace minerals that support the proliferation of fibroblast cells. To promote adherence to the cell culture bottom, the supplement contains 2% FBS.
Quality Control
Each lot of Glioblastoma Cell Growth Medium undergoes a rigorous quality control process to ensure it meets the highest standards. The pH of the medium is maintained at 7.2 +/
- 0.02 at 20-25°C. Each lot is tested for sterility and the absence of mycoplasma and bacteria. This ensures that the medium is free from contamination and will provide consistent results.
Storage and Maintenance

Glioblastoma Cell Growth Medium should be stored refrigerated at +2°C to +8°C in the dark.
Freezing or warming up to +37°C can compromise the quality of the product.
To use the medium, remove the required amount from the bottle and warm it to room temperature. The shelf life of the product is eight weeks from the date of manufacture.

Disclaimer
Glioblastoma Cell Growth Medium is for in-vitro use only and is not intended for clinical or diagnostic applications. It is designed to be used by trained professionals and researchers in a laboratory setting.

This medium is defined, free of serum or Xeno additives and based on the formulation of the E6 medium but modified for increased performance of iPSCs. iPSC growth medium represents a cell culture medium that is suited for the propagation of human induced pluripotent stem cells of various origins. Upon supplementation, it should be used up within 2 weeks due to limited shelf life. It is recommended to prepare the medium for cell culture in aliquots unless the volume of 500 ml will be used within this time range. This medium is tested with iPS cells cultured on Vitronectin and iMatrix-coated 6-well-plates.

iPSC colonies

Figure 1: Examples of induced-pluripotent cells, iPSC-ASC (left) and iPSC-hDPSC (right), the latter derived by reprogramming Mesenchymal Stromal cells isolated from human Dental Pulp cells, cultivated in the CLS iPSC growth medium.

Pluripotency of cells cultured in iPSC growth medium
1. Flow cytometry data

Figure 2: Pluripotency of iPSC-hDPSC (clone 2) by flow cytometry: the cells were propagated in iPSC growth medium in 6-well-plates, detached using Accutase and stained. A) SOX-2, B) Oct3-4, C) Nanog.

2. Immunocytochemistry

Figure 3: Pluripotency of iPSC-hDPSC (clone 1) by immunocytochemical staining: iPSC-hDPSC derived by reprogramming Mesenchymal Stromal cells isolated from human Dental Pulp cells, cultivated in the iPSC growth medium in glass bottom dishes (ibidi). iPSCs expressed the pluripotency markers Oct3/4, SSEA4, TRA-60-1 and SOX2, staining was conducted using the PSC Immunocytochem Kit 4-Marker (Thermofisher).
Media preparation

The supplement comes in two parts, the basic medium and the supplement comprising very sensitive ingredients.
Due to the reduced shelf life of the media once supplemented, it is recommended to prepare smaller quantities such as 100 mL.
To prepare 100 mL of the iPSC growth medium, thaw the supplement at room temperature (18-25°C) or overnight at 4-8°C. Do not thaw at 37°C in a water bath, as this may be harmful to the ingredients. Aliquot the remaining 40 mL adequately and freeze down immediately.
Aseptically transfer 90 mL of the iPSC-MG basic medium into a sterile 100 mL bottle and add 10 mL of the supplement. Mix thoroughly. This volume should be used up within 1-2 weeks.
If other volumes of media are required, prepare accordingly.
For sterile filtration purposes, use 0.2 µm low protein binding polyethersulfone (PES) filter units (e.g. Corning Cat # 431229).

Maintenance

Keep the basic medium and the supplemented medium refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimizes the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Store the supplement at -20°C. Do not leave it for a prolonged time at ambient temperature.
After supplementation, this medium has a shelf life of about two weeks.

The BT-474 Cell Line Medium is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Ham's F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. Furthermore, it is an excellent basic medium for the serum free cell culture. It was supplemented with insulin to support the proliferation of BT-474 cells.

SF-9 Cell Growth Medium is a serum
- and protein-free medium optimized for the growth of insect cells like Sf9 and Sf21 (Spodoptera frugiperda). SF-9 Cell
Growth

Medium has been developed for the cultivation of insect cells and is suitable for the production of recombinant proteins (Baculovirus expression vector system).
Usage instructions
You can follow this protocol to adapt your insect cell line to protein-free culture in a serum-free medium, for SF-9 cells. The optimal temperature range for most insect cells is 25°C to 30°C (27°C incubation +/
- 0.5°C). The pH for cell culture with Sf9, and Sf21 should be between pH 6.0 and pH 6.4, and the osmolality should be 345-380 mOsm/kg. For oxygen supply, use vessels with filter lids or slightly unscrew the lids. For adaption from serum-containing to protein-free culture, direct or sequential adaption can be done. Cells should be taken from the middle exponential growth phase with >90% viability.
Direct adaptation

Transfer cells from serum-containing culture directly into SF-9 Cell
Growth

Medium (prewarmed to ambient temperature) with a density of 0.5 million cells/ml.
Subculture cells at > 2 million cells/ml, seed into fresh SF-9 Cell
Growth

Medium with a density of 0.5 million cells/ml.
Repeat the subculture routine till the cells reach > 80% viability.

Indirect adaptation

Start with subculturing the cells into a 1+1 mixture of the serum-containing medium and serum-free medium, for SF-9 cells, with a density of 0.5 million cells/ml.
At > 1 million cells/ml, dilute to 0.5 million cells/ml by adding the respective volume of serum-free medium, for SF-9 cells.
Repeat this process till serum levels below 0.1% is reached; the viability should be above 80%. Cell density should exceed 1 million cells/ml.

Quality control

pH = 7.2 +/
- 0.02 at 20-25°C
Each lot has been tested for sterility and absence of mycoplasma and bacteria

Maintenance

Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimizes the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up at room temperature.
Shelf life for any medium except for the basic medium is 8 weeks from the date of manufacture.

Ingredients
SF-9 Cell
Growth

Medium contains amino acids, vitamins, salts, trace elements, lipids, and growth-promoting factors in a formulation optimized for insect cells. It is free of proteins and any components of human origin.
Disclaimer
This product is for in-vitro use only. Not intended for clinical or diagnostic applications.

In-Vitro Research and Development: The Role of Endothelial Cell Growth Medium
Endothelial cells play a crucial role in the circulatory system by transporting nutrients and waste throughout the body. Researchers and scientists need a medium that can sustain the development and maintenance of endothelial cells in vitro so that they may study their function and malfunction. CLS Endothelial Cell Growth Medium fills this need by creating an environment where endothelial cells may thrive throughout their growth and maintenance phases.
Definition of Endothelial Cell Growth Media
An in-vitro cell culture system requires a particular solution called Endothelial Cell Growth Media to sustain the endothelial cells necessary for the experiment. Endothelial cells in the body are exposed to an environment mimicked by the medium, including necessary and non-essential amino acids, vitamins, hormones, growth factors, and trace minerals. This nutrient-dense fluid is critical for preserving the endothelium phenotype and function because it stimulates cell proliferation. Globally, researchers and scientists rely on the CLS Endothelial Cell Growth Medium because it is both effective and simple.
Importance of Endothelial Cell Research
Blood artery creation, blood pressure control, and wound healing are just a few physiological processes in which endothelial cells play a key part. To keep the blood vessels balanced, these cells may react to chemical and mechanical stimuli. In order to fully comprehend the causes of hypertension, atherosclerosis, and thrombosis, research into endothelial cells is crucial. Investigating endothelial cells may also provide light on how to treat these diseases medically. Providing a stable and uniform environment for endothelial cell development and proliferation, the CLS Endothelial Cell Growth Medium has shown to be an indispensable tool in this kind of study.
Quality Assurance
High-quality in-vitro cell culture media is essential for obtaining consistent and repeatable outcomes. Quality control testing is performed on every CLS Endothelial Cell Growth Media batch to guarantee its uniformity and effectiveness. The sterility, lack of mycoplasma and bacteria, and pH levels of each batch are examined. To promote healthy cell development and proliferation, the medium's pH is kept at 7.2 +/
- 0.02, and the temperature is maintained between 20 and 25 degrees Celsius. To ensure the culture is clear of any contamination that might impact the accuracy of the findings, the medium is also checked for microbiological pollutants such as fungus, bacteria, and mycoplasma.
Maintenance and Disclaimer
The expiration date of CLS Endothelial Cell Growth Media is six weeks from the production date. The medium should be kept at a temperature of between +2°C and +8°C, away from light, and never frozen or heated over 37°C to preserve its quality. As a corollary, it is crucial to avoid microwaves and other unregulated heat sources that might compromise the product's integrity. A portion of the medium may be taken out of the container and brought up to room temperature if just a little amount will be utilized. CLS's Endothelial Cell Growth Medium is a tried and true method for cultivating endothelial cells because of the stringent quality controls used in its production.
Notably, the CLS Endothelial Cell Growth Medium is not for clinical or diagnostic use; it is designed primarily for in-vitro usage. Obtaining accurate and reliable findings from the medium requires strict adherence to the manufacturer's guidelines and quality control methods.
Applications of the Medium
The CLS Endothelial Cell Growth Medium has proven useful in several biological studies. As this medium may be used to cultivate endothelial cells, it can be used to simulate endothelial function and malfunction in organ systems in vitro. This is especially useful for studying the blood-brain barrier or tissue-engineered blood arteries. This is essential for understanding disease processes and creating effective treatments.
Endothelial cells, especially HUVEC cells, have been extensively employed in angiogenesis research, wound healing research, and cancer research, thus, the medium has also been modified to promote their proliferation. Accurate and repeatable findings depend on the consistency and high quality of the medium used to develop these cells, and the CLS Endothelial Cell Growth Media meets both of these requirements.
Conclusion
Researchers and professionals in the field of biomedical engineering may benefit significantly from using the CLS Endothelial Cell Growth Media. For tissue engineering and drug discovery studies, the medium is highly recommended since it promotes the growth of endothelial cells by simulating the endothelial cell environment seen in the body. The medium is very simple to use, needs no unique care, and is subjected to stringent quality assurance procedures to guarantee its efficacy.
Research and knowledge of endothelial function and malfunction may significantly influence the development of therapeutics for atherosclerosis and associated disorders, and the endothelial cell culture medium is a crucial instrument in this endeavor. This medium has become a vital instrument for accomplishing research aims and advancing scientific progress since it provides excellent cell proliferation and maintenance conditions.
To sum up, the CLS Endothelial Cell Growth Medium is a reliable option for scientists studying endothelial cells for their potential in biomedicine.

FRTL Cell Growth medium is based on Ham’s F12K medium and supplemented with hormones, L-Glutamine and FBS. Together with the TSH stock solution coming with the medium, it is ideally suited for propagating FRTL and FRTL-5 cells. Directly before usage, add the respective amount of TSH. Each aliquot provides enough TSH for preparing 100 ml complete FRTL Cell Growth Medium with a final TSH concentration of 16.5 ng/ml.
Cell lines cultured using FRTL Cell Growth Medium

Phase contrast images of FRTL-5 cells: FRTL-5 cells growing in FRTL Cell Growth Medium without TSH (right) and supplemented with 16.5 ng/mL TSH (left) 8 days after seeding.
Medium preparation

The FRTL Growth Medium is a basic medium, which must be finalized by adding the supplement mix provided in frozen 15 mL tubes.
It is recommended to prepare 100 mL of medium. Take out 90 mL basic medium and add 10 mL of supplements from one of the 5 tubes, defrosted at 37°C for a maximum of 5-10 minutes. Although the supplement mix is a sterile solution, we recommend filtering it through a sterile 0.2 µm pore PES membrane syringe filter. The volume of 100 mL is sufficient to start culturing the cells and for the first subculture steps.
Adding 10 mL supplement mix to 90 mL basic medium will result in a TSH final concentration of 16.5 ng/mL.

The proliferation of FRTL-5 cells depends on the presence of TSH in the cell culture medium

TSH-dependent FRTL-5 cell numbers. FRTL-5 were seeded at the same cell density in a medium without TSH (blue bars), containing increasing TSH concentrations (orange bars), and harvested after 8 days of incubation at 37°C/5% CO2. Cells were counted using the DeNovix Celldrop electronic cell counter in the presence of Trypan Blue.
Maintenance

Basic Medium: Keep refrigerated at +2°C to +8°C in the dark. Freezing and warming up to +37° C minimize the quality of the product. Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g., microwave appliances).
Supplements: Keep frozen at -20°C in the dark.

Quality control

pH = 7.2 +/
- 0.02 at 20-25°C.
Each lot has been tested for sterility and the absence of mycoplasma and bacteria.

The NCI-H295R Cell Growth Medium is a 1:1 mixture of DMEM containing 4.5 g/L glucose and Hams F12. As this medium is rich in amino acids, hormones and minerals, it supports the growth of many cells and cell lines even under low serum conditions. Thus, it is possible to work with serum concentrations of 5% or less. This special medium has been adapted to support the proliferation of NCI-H295R cells. The final concentration of bovine serum is below 1%.

Comprehensive Guide to Fibroblast Cell Growth Media for Cell Culture Research
In the field of cell culture research, our Fibroblast Cell Growth Medium has evolved into a powerful instrument for the investigation of numerous illnesses and ailments. Being a complete medium intended exclusively for the culture of fibroblasts, it is prepared to assist the development and multiplication of fibroblast cells, which are present in several human tissues.
What does Fibroblast Cell Growth Media consist of?
Our Fibroblast Cell Growth Medium is a clear, transparent liquid with a pH range of 6-8 that comprises a mix of dissolved inorganic salts, amino acids, vitamins, carbohydrates, growth factors, hormones, and trace minerals. The medium is intended to supply the components required for the development and multiplication of fibroblast cells, which are crucial for a variety of scientific applications.
Why are fibroblasts essential to scientific research?
Many illnesses and ailments, such as cancer, arthritis, and fibrosis, are being studied using fibroblasts, which have become a vital tool. In addition, they are utilized to create induced pluripotent stem cells (iPSCs), which have the potential to revolutionize regenerative medicine.
Fibroblast Cell Growth Medium Ingredients
Our Fibroblast Cell Growth Medium comprises amino acids, vitamins, organic and inorganic chemicals, hormones, growth factors, and trace minerals, both essential and non-essential. Moreover, 2% Fetal Bovine Serum (FBS) is added to the medium to increase cell adhesion to the culture plate.
The medium is evaluated and adjusted for use in an incubator with 5% CO2 and 95% air. Each batch of the medium is checked for sterility, lack of mycoplasma, and presence of bacteria to assure its quality and uniformity.
Administration and Storage

To preserve the product's quality, the medium should be kept refrigerated between +2°C and +8°C in the dark.
The medium should not be heated over 37°C or subjected to unregulated heat sources.
Freezing or warming up to +37°C might diminish the quality of the product (e.g., microwave appliances).
If just a portion of the medium will be used, it must be withdrawn from the container and brought to room temperature.
The product has a six-week shelf life from the date of manufacturing and is designed only for in-vitro usage, not for clinical or diagnostic use.